MOSPD2 Polyclonal antibody

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• MOSPD2 Polyclonal antibody (25617-1-AP)

• 

  WB analysis using 25617-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25617-1-AP (MOSPD2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 25617-1-AP

  WB result of MOSPD2 antibody (25617-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MOSPD2 transfected HeLa cells.

• Featured Product
• KD/KO Validated

MOSPD2 Polyclonal Antibody for WB, ELISA

Cat No. 25617-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Motile sperm domain-containing protein 2

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Tested Applications

• Positive WB detected in: HuH-7 cells, HeLa cells, J774A.1 cells, RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25617-1-AP targets MOSPD2 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag22206

  Product name: Recombinant human MOSPD2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 64-496 aa of BC030641

  Sequence: MLDESFQWRKEISVNDLNESSIPRWLLEIGVIYLHGYDKEGNKLFWIRVKYHVKDQKTILDKKKLIAFWLERYAKRENGKPVTVMFDLSETGINSIDMDFVRFIINCFKVYYPKYLSKIVIFDMPWLMNAAFKIVKTWLGPEAVSLLKFTSKNEVQDYVSVEYLPPHMGGTDPFKYSYPPLVDDDFQTPLCENGPITSEDETSSKEDIESDGKETLETISNEEQTPLLKKINPTESTSKAEENEKVDSKVKAFKKPLSVFKGPLLHISPAEELYFGSTESGEKKTLIVLTNVTKNIVAFKVRTTAPEKYRVKPSNSSCDPGASVDIVVSPHGGLTVSAQDRFLIMAAEMEQSSGTGPAELTQFWKEVPRNKVMEHRLRCHTVESSKPNTLTLKDNAFNMSDKTSEDICLQLSRLLESNRKLEDQVQRCIWFQQ

• Full Name: motile sperm domain containing 2
• Calculated Molecular Weight: 518 aa, 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC030641
• Gene Symbol: MOSPD2
• Gene ID (NCBI): 158747
• RRID: AB_3742155
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NHP6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MOSPD2 is a 518-amino acid protein containing an N-terminal CRAL/TRIO domain, a major sperm protein (MSP) domain, and a potential C-terminal transmembrane domain. MOSPD2 is a member of the vesicle-associated membrane protein-associated protein (VAP) family and one of the endoplasmic reticulum (ER) receptors that function as tether proteins to bridge ER and other organelles to build contacts(PMID: 29858488, 35389430).