MOV10 Recombinant antibody

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  WB analysis using 85818-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85818-5-RR (MOV10 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 85818-5-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MOV10 antibody (85818-5-RR, Clone: 250130D2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 85818-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85818-5-RR against Human MOV10 were performed. The affinity constant is below 1 pM.

MOV10 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 85818-5-RR

Clone No.250130D2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Armitage homolog, EC:3.6.4.13, fSAP113, gb110, Helicase MOV-10

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, HEK-293 cells, L02 cells, MCF-7 cells, NIH/3T3 cells, C6 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85818-5-RR targets MOV10 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MOV10 fusion protein Ag0384
• Full Name: Mov10, Moloney leukemia virus 10, homolog (mouse)
• Calculated Molecular Weight: 114 kDa
• Observed Molecular Weight: 115 kDa
• GenBank Accession Number: BC002548
• Gene Symbol: MOV10
• Gene ID (NCBI): 4343
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9HCE1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MOV10, also named as KIAA1631, belongs to the DNA2/NAM7 helicase family and SDE3 subfamily. It is required for RNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Human MOV10 may regulate a wide range of RNA viruses and could also control the retrotransposition of endogenous retroelements in mammals (PMID:22727223). MOV10 has a broad antiretroviral activity that can target a wide range of retroviruses, and it could be actively involved in host defense against retroviral infection. MOV10 can potently inhibit HIV-1 replication at multiple stages (PMID:20215113). It is involved in the progression of telomerase-catalyzing reaction via the interaction of telomerase protein and telomere DNA.