MOXD1 Polyclonal antibody

MOXD1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11545-1-AP

Synonyms

PRO5780, MOX, monooxygenase, DBH like 1, Monooxygenase X, EC:1.14.17.-

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Validation Data Gallery

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  WB analysis using 11545-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11545-1-AP (MOXD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 11545-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11545-1-AP (MOXD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 11545-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11545-1-AP (MOXD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 11545-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11545-1-AP (MOXD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 11545-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11545-1-AP (MOXD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 11545-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MOXD1 antibody (11545-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 11545-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MOXD1 antibody (11545-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

11545-1-AP targets MOXD1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MOXD1 fusion protein Ag2060
• Full Name: monooxygenase, DBH-like 1
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 63-70 kDa
• GenBank Accession Number: BC018756
• Gene Symbol: MOXD1
• Gene ID (NCBI): 26002
• RRID: AB_10638475
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Monooxygenase DBH like 1 (MOXD1) is a member of the copper monooxygenase family, including dopamine monooxygenase (dopamine β-monooxygenase, DBM) and peptidylglycine α-hydroxylating monooxygenase (PHM). MOXD1 is expressed during the development of the neural crest, and distributed along the olfactory bulb, cerebellum, brain stem, and parietal cortex with the development of nerve cells, participating in the basal plate. MOXD1 is highly expressed in GBM cells and can promote the growth of cancer cells (PMID: 35393406). In addition, MOXD1 is a gate-keeper of organ homeostasis and functions as a tumor-suppressor in neuroblastoma

Publications

Species	Application	Title
mouse	WB	Funct Integr Genomics; Depletion of PIEZO1 expression is accompanied by upregulating p53 signaling in mice with perioperative neurocognitive disorder; Authors - Fuhai Bai
human,mouse	WB,IHC	Life Sci; Fenofibrate inhibits MOXD1 and PDZK1IP1 expression and improves lipid deposition and inflammation in mice with alcoholic fatty liver; Authors - Tongtong Pan