MPG Monoclonal antibody

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  WB analysis using 67920-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67920-1-Ig (MPG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis using 67920-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67920-1-Ig (MPG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

MPG Monoclonal Antibody for WB, ELISA

Cat No. 67920-1-Ig

Clone No.2C12F3

Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat and More (1)

Applications

WB, ELISA

Synonyms

3 alkyladenine DNA glycosylase, AAG, ADPG, anpg, APNG, CRA36.1, MDG, Mid1, MPG, N methylpurine DNA glycosylase, PIG11, PIG16

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Tested Applications

• Positive WB detected in: HeLa cells, HSC-T6 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, PC-12 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

67920-1-Ig targets MPG in WB, ELISA applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28647

  Product name: Recombinant human MPG protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-293 aa of BC014991

  Sequence: MPARSGAQFCRRMGQKKQRPARAGQPHSSSDAAQAPAEQPHSSSDAAQAPCPRERCLGPPTTPGPYRSIYFSSPKGHLTRLGLEFFDQPAVPLARAFLGQVLVRRLPNGTELRGRIVETEAYLGPEDEAAHSRGGRQTPRNRGMFMKPGTLYVYIIYGMYFCMNISSQGDGACVLLRALEPLEGLETMRQLRSTLRKGTASRVLKDRELCSGPSKLCQALAINKSFDQRDLAQDEAVWLERGPLEPSEPAVVAAARVGVGHAGEWARKPLRFYVRGSPWVSVVDRVAEQDTQA

• Full Name: N-methylpurine-DNA glycosylase
• Calculated Molecular Weight: 293 aa, 32 kDa
• Observed Molecular Weight: 35-36 kDa
• GenBank Accession Number: BC014991
• Gene Symbol: MPG
• Gene ID (NCBI): 4350
• RRID: AB_2918672
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P29372
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Sci Adv; Deficiency in mammalian STN1 promotes colon cancer development via inhibiting DNA repair; Authors - Dinh Duc Nguyen