MPG Monoclonal antibody

MPG Monoclonal Antibody for WB, ELISA

Cat No. 67920-1-Ig

Clone No.2C12F3

Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat and More (1)

Applications

WB, ELISA

Synonyms

3 alkyladenine DNA glycosylase, AAG, ADPG, anpg, APNG, CRA36.1, MDG, Mid1, MPG, N methylpurine DNA glycosylase, PIG11, PIG16

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWB

WB analysis using 67920-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67920-1-Ig (MPG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 67920-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67920-1-Ig (MPG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive WB detected in

HeLa cells, HSC-T6 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, PC-12 cells

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

67920-1-Ig targets MPG in WB, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity	Human, rat
Cited Reactivity	human
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag28647 Product name: Recombinant human MPG protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-293 aa of BC014991 Sequence: MPARSGAQFCRRMGQKKQRPARAGQPHSSSDAAQAPAEQPHSSSDAAQAPCPRERCLGPPTTPGPYRSIYFSSPKGHLTRLGLEFFDQPAVPLARAFLGQVLVRRLPNGTELRGRIVETEAYLGPEDEAAHSRGGRQTPRNRGMFMKPGTLYVYIIYGMYFCMNISSQGDGACVLLRALEPLEGLETMRQLRSTLRKGTASRVLKDRELCSGPSKLCQALAINKSFDQRDLAQDEAVWLERGPLEPSEPAVVAAARVGVGHAGEWARKPLRFYVRGSPWVSVVDRVAEQDTQA Predict reactive species
Full Name	N-methylpurine-DNA glycosylase
Calculated Molecular Weight	293 aa, 32 kDa
Observed Molecular Weight	35-36 kDa
GenBank Accession Number	BC014991
Gene Symbol	MPG
Gene ID (NCBI)	4350
RRID	AB_2918672
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P29372
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.