Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, MCF-7 cells, U-251 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16796-1-AP targets MPP8 in WB, IHC, IF/ICC, IP, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10268 Product name: Recombinant human MPHOSPH8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-301 aa of BC003542 Sequence: MEQVAEGARVTAVPVSAADSTEELAEVEEGVGVVGEDNDAAARGAEAFGDSEEDGEDVFEVEKILDMKTEGGKVLYKVRWKGYTSDDDTWEPEIHLEDCKEVLLEFRKKIAENKAKAVRKDIQRLSLNNDIFEANSDSDQQSETKEDTSPKKKKKKLRQREEKSPDDLKKKKAKAGKLKDKSKPDLESSLESLVFDLRTKKRISEAKEELKESKKPKKDEVKETKELKKVKKGEIRDLKTKTREDPKENRKTKKEKFVESQVESESSVLNDSPFPEDDSEGLHSDSREEKQNTKSARERAG Predict reactive species |
| Full Name | M-phase phosphoprotein 8 |
| Calculated Molecular Weight | 860 aa, 97 kDa |
| Observed Molecular Weight | 97-105 kDa |
| GenBank Accession Number | BC003542 |
| Gene Symbol | MPP8 |
| Gene ID (NCBI) | 54737 |
| RRID | AB_2266644 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99549 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The M-phase phosphoprotein 8 (MPP8) was firstly identified to coimmunoprecipitate with the RanBPM-comprised large protein complex [PMID:19956676]. It also binds to methylated H3K9 and recruited the H3K9 methyltransferases GLP and ESET, as well as DNA methyltransferase 3A (DNMT3A) to the promoter of the E-cadherin gene, a key regulator of tumor cell growth and epithelial-to-mesenchymal transition (EMT) [PMID:18726070]. This binding event of MPP8-H3K9me modulates the expression of E-cadherin, a key regulator of cell-cell adhesion with implications in embryonic development, tissue morphogenesis and homeostasis , together with H3K9 methyltransferases GLP and ESET and DNA methyltransferase 3a (Dnmt3a) [PMID: 18726070].
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MPP8 antibody 16796-1-AP | Download protocol |
| IP protocol for MPP8 antibody 16796-1-AP | Download protocol |
| WB protocol for MPP8 antibody 16796-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MPP8 antibody (Cat# 16796-1-AP) has 37 citations spanning journals including Nature, Science, Cell, Nature Genetics, Nature Communications, Molecular Cell, Nucleic Acids Research, Cell Reports, and others. Associated research fields cover epigenetics, gene silencing, HUSH complex function, transposable element regulation, chromatin biology, stem cell biology, cancer, and virology.
| Species | Application | Title |
|---|---|---|
Science GENE SILENCING. Epigenetic silencing by the HUSH complex mediates position-effect variegation in human cells.
| ||
Nat Genet Silencing of LINE-1 retrotransposons is a selective dependency of myeloid leukemia. | ||
Nat Genet Hyperactivation of HUSH complex function by Charcot-Marie-Tooth disease mutation in MORC2. | ||
Nat Genet Promoter strength and position govern promoter competition through transcript-dependent insulation. |
Reviews
What customers say
All three reviews are 5-star ratings. Users consistently describe the antibody as excellent and reliable for Western Blot and Immunoprecipitation. It was tested successfully in U2OS, mESC, HeLa, and Jurkat cells. One reviewer highlighted its strong performance for IP of MPP8, noting it works well on WB at a 1:1000 dilution with a 1-hour incubation at room temperature. Overall, reviewers express high satisfaction with the antibody's specificity and ease of use across multiple cell types.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Tsimafei (Verified Customer) (09-22-2025) | Excellent antibody!
 |
Julia (Verified Customer) (08-04-2025) | Good antibody! works great for mESC
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Roy (Verified Customer) (06-12-2024) | Great antibody to IP MPP8, working well on WB as well (1/1000, 1h at RT) in HeLa cells and Jurkat cells.
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