Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells, MDA-MB-231 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12983-1-AP targets MPP7 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3612 Product name: Recombinant human MPP7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 269-576 aa of BC038105 Sequence: DATWWQAKHEADANPRAGLIPSKHFQERRLALRRPEILVQPLKVSNRKSSGFRRSFRLSRKDKKTNKSMYECKKSDQYDTADVPTYEEVTPYRRQTNEKYRLVVLVGPVGVGLNELKRKLLISDTQHYGVTVPHTTRARRSQESDGVEYIFISKHLFETDVQNNKFIEYGEYKNNYYGTSIDSVRSVLAKNKVCLLDVQPHTVKHLRTLEFKPYVIFIKPPSIERLRETRKNAKIISSRDDQGAAKPFTEEDFQEMIKSAQIMESQYGHLFDKIIINDDLTVAFNELKTTFDKLETETHWVPVSWLHS Predict reactive species |
| Full Name | membrane protein, palmitoylated 7 (MAGUK p55 subfamily member 7) |
| Calculated Molecular Weight | 576 aa, 66 kDa |
| Observed Molecular Weight | 66 kDa |
| GenBank Accession Number | BC038105 |
| Gene Symbol | MPP7 |
| Gene ID (NCBI) | 143098 |
| RRID | AB_2291710 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5T2T1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MPP7 antibody 12983-1-AP | Download protocol |
| IP protocol for MPP7 antibody 12983-1-AP | Download protocol |
| WB protocol for MPP7 antibody 12983-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-MPP7 antibody (Cat# 12983-1-AP) has 9 citations published in journals including EMBO Reports, Toxicological Sciences, Scientific Reports, Life, Cell Biology International, Calcified Tissue International, Journal of Cancer, Human Molecular Genetics, and bioRxiv. Research fields span muscle stem cell biology, ALS-linked TDP-43 pathology, allergic airway inflammation, esophageal and breast cancer, osteogenesis and bone mineralization, ovarian cancer EMT, bone density genetics, and RNA–small molecule interactions.
| Species | Application | Title |
|---|---|---|
Sci Rep MAGUK p55 subfamily member 7 attenuates allergic airway inflammation by modulating lung dendritic cells functions. | ||
Life (Basel) MPP7 as a Novel Biomarker of Esophageal Cancer: MPP7 Knockdown Inhibits Esophageal Cancer Cell Migration and Invasion
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Calcif Tissue Int Membrane Palmitoylated Protein 7 is Required for Osteogenesis and is Linked with Bone Mineralization and Osteoporosis: The Functional Evaluation of GEFOS GWAS Hit.
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Cell Biol Int MPP7 promotes the migration and invasion of breast cancer cells via EGFR/AKT signaling.
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