MPPED1 Polyclonal antibody

MPPED1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13677-1-AP

Synonyms

239AB, Adult brain protein 239, C22orf1, FAM1A, MPPED1

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Validation Data Gallery

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  WB analysis of mouse brain using 13677-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13677-1-AP (MPPED1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 13677-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 13677-1-AP (MPPED1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas using 13677-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 13677-1-AP (MPPED1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 13677-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 13677-1-AP (MPPED1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 13677-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13677-1-AP (MPPED1 Antibody) at dilution of 1:50(under 10x lens).

• 

  IHC staining of human heart using 13677-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13677-1-AP (MPPED1 Antibody) at dilution of 1:50(under 40x lens).

• 

  IHC staining of human brain using 13677-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13677-1-AP (MPPED1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 13677-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13677-1-AP (MPPED1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 13677-1-AP

  Immunofluorescent analysis of Hela cells, using MPPED1 antibody 13677-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: human pancreas tissue, human brain tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13677-1-AP targets MPPED1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MPPED1 fusion protein Ag4672
• Full Name: metallophosphoesterase domain containing 1
• Calculated Molecular Weight: 326 aa, 37 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC028035
• Gene Symbol: MPPED1
• Gene ID (NCBI): 758
• RRID: AB_10644336
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.