MPPED2 Polyclonal antibody

MPPED2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13270-1-AP

Synonyms

Metallophosphoesterase domain-containing protein 2, FAM1B, EC:3.1.-.-, C11orf8, 239FB

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Validation Data Gallery

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  WB analysis of human brain using 13270-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13270-1-AP (MPPED2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 13270-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13270-1-AP (MPPED2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 13270-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13270-1-AP (MPPED2 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 13270-1-AP

  Immunofluorescent analysis of HepG2 cells, using MPPED2 antibody 13270-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HeLa using 13270-1-AP

  Immunofluorescent analysis of Hela cells, using MPPED2 antibody 13270-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of HeLa using 13270-1-AP

  Immunofluorescent analysis of Hela cells, using MPPED2 antibody 13270-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: human brain tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13270-1-AP targets MPPED2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MPPED2 fusion protein Ag3963
• Full Name: metallophosphoesterase domain containing 2
• Calculated Molecular Weight: 294 aa, 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC031582
• Gene Symbol: MPPED2
• Gene ID (NCBI): 744
• RRID: AB_2144517
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.