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MPRIP Polyclonal antibody, PBS Only (20040-1-PBS)

Filter: AllWBIPIHCIF-FroIF/ICC

WB analysis of SH-SY5Y using 20040-1-AP (same clone as 20040-1-PBS)
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20040-1-AP (MPRIP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20040-1-PBS in a different storage buffer formulation.
WB analysis of SKOV-3 using 20040-1-AP (same clone as 20040-1-PBS)
SKOV-3 cells were subjected to SDS PAGE followed by western blot with 20040-1-AP (MPRIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20040-1-PBS in a different storage buffer formulation.
WB analysis of A2780 using 20040-1-AP (same clone as 20040-1-PBS)
A2780 cells were subjected to SDS PAGE followed by western blot with 20040-1-AP (MPRIP Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20040-1-PBS in a different storage buffer formulation.
IP experiment of SKOV-3 using 20040-1-AP (same clone as 20040-1-PBS)
IP result of anti-MPRIP (IP:20040-1-AP, 4ug; Detection:20040-1-AP 1:500) with SKOV-3 cells lysate 2000ug. This data was developed using the same antibody clone with 20040-1-PBS in a different storage buffer formulation.
IHC staining of human ovary using 20040-1-AP (same clone as 20040-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary using 20040-1-AP (MPRIP antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20040-1-PBS in a different storage buffer formulation.
IHC staining of human ovary using 20040-1-AP (same clone as 20040-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary using 20040-1-AP (MPRIP antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20040-1-PBS in a different storage buffer formulation.
IHC staining of mouse ovary using 20040-1-AP (same clone as 20040-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 20040-1-AP (MPRIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20040-1-PBS in a different storage buffer formulation.
IF Staining of mouse brain using 20040-1-AP (same clone as 20040-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed frozen OCT-embedded mouse brain tissue using 20040-1-AP (MPRIP antibody) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20040-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 20040-1-AP (same clone as 20040-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MPRIP antibody (20040-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 20040-1-PBS in a different storage buffer formulation.

MPRIP Polyclonal antibody, PBS Only

Name: MPRIP Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 20040-1-PBS

MPRIP Polyclonal Antibody for WB, IHC, IF/ICC, IF-Fro, IP, Indirect ELISA

Cat No. 20040-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-Fro, IP, Indirect ELISA

Synonyms

M RIP, MRIP, M-RIP, Myosin phosphatase Rho-interacting protein, p116Rip

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

20040-1-PBS targets MPRIP in WB, IHC, IF/ICC, IF-Fro, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	myosin phosphatase Rho interacting protein
Calculated Molecular Weight	117 kDa
Observed Molecular Weight	116-140 kDa
GenBank Accession Number	NM_015134
Gene Symbol	MPRIP
Gene ID (NCBI)	23164
RRID	AB_10694433
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q6WCQ1
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

MPRIP, also named as KIAA0864, MRIP, RHOIP3 and p116Rip, is required for the regulation of the actin cytoskeleton by RhoA and ROCK1. MRPIP targets myosin phosphatase to the actin cytoskeleton. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells. The antibody recognizes the C-term of isoform2 and isoform3 of MPRIP.