MPRIP Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 86033-1-RR (same clone as 86033-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86033-1-RR (MPRIP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86033-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 86033-1-RR (same clone as 86033-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using MPRIP antibody (86033-1-RR, Clone: 250643B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86033-1-PBS in a different storage buffer formulation.

MPRIP Uni-rAb^® Recombinant Antibody for WB, IF-P, Indirect ELISA

Cat No. 86033-1-PBS

Clone No.250643B2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF-P, Indirect ELISA

Synonyms

M RIP, MRIP, M-RIP, Myosin phosphatase Rho-interacting protein, p116Rip

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Product Information

86033-1-PBS targets MPRIP in WB, IF-P, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: myosin phosphatase Rho interacting protein
• Calculated Molecular Weight: 117 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: NM_015134
• Gene Symbol: MPRIP
• Gene ID (NCBI): 23164
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q6WCQ1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MPRIP, also named as KIAA0864, MRIP, RHOIP3 and p116Rip, is required for the regulation of the actin cytoskeleton by RhoA and ROCK1. MRPIP targets myosin phosphatase to the actin cytoskeleton. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells. The antibody recognizes the C-term of isoform2 and isoform3 of MPRIP.