MRAP Polyclonal antibody

MRAP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 19512-1-AP

Synonyms

B27, C21orf61, FALP, FGD2, GCCD2, MRAP

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Validation Data Gallery

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  WB analysis of mouse kidney using 19512-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19512-1-AP (MRAP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 19512-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19512-1-AP (MRAP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 19512-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19512-1-AP (MRAP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse kidney using 19512-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney using 19512-1-AP (MRAP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse kidney using 19512-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney using 19512-1-AP (MRAP antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 19512-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 19512-1-AP (MRAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse kidney tissue
• Positive IHC detected in: human testis tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

19512-1-AP targets MRAP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: melanocortin 2 receptor accessory protein
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 12 kDa
• GenBank Accession Number: NM_178817
• Gene Symbol: MRAP
• Gene ID (NCBI): 56246
• RRID: AB_10694272
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MRAP, also named as C21orf61, FALP and B27, is required for MC2R expression in certain cell types. MRAP is involved in the processing, trafficking or function of MC2R. MRAP may be involved in the intracellular trafficking pathways in adipocyte cells. Mutation of MRAP will cause glucocorticoid deficiency type 2 (GCCD2) which known as familial glucocorticoid deficiency type 2 (FGD2). The antibody can recognize the isoform1,isoform2 and isoform4.

Publications

Species	Application	Title
rat	WB	Cell Tissue Res; ACTH promotes chondrogenic nodule formation and induces transient elevations in intracellular calcium in rat bone marrow cell cultures via MC2-R signaling.; Authors - Jodi F Evans