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CD206 Monoclonal antibody
CD206 Monoclonal Antibody for IHC, IP, WB, ELISA
Cat no : 60143-1-Ig
Validation Data Gallery
|Positive WB detected in||human placenta tissue, human liver tissue|
|Positive IP detected in||human placenta tissue|
|Positive IHC detected in||human lung cancer tissue, human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:10000-1:40000|
|Sample-dependent, check data in validation data gallery|
60143-1-Ig targets CD206 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, pig|
|Host / Isotype||Mouse / IgG2a|
|Full Name||mannose receptor, C type 1|
|Calculated molecular weight||166 kDa|
|Observed molecular weight||170 kDa|
|GenBank accession number||NM_002438|
|Gene ID (NCBI)||4360|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CD206, also named as MMR, CLEC13D and MRC1, is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. CD206 has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment. CD206 is a 170 kDa transmembrane protein which contains 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. It is expressed on most tissue macrophages, in vitro derived dendritic cells, lymphatic and sinusoidal endothelia. This antibody recognizes the intracellular carboxy-terminal part of CD206 and MRC1L1.
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