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CD206 Monoclonal antibody
CD206 Monoclonal Antibody for WB, IHC, IF-P, IP, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human and More (1)
Applications
WB, IHC, IF-P, IP, ELISA
Conjugate
Unconjugated
163
CloneNo.
2A6A10
Cat no : 60143-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human placenta tissue, human liver tissue |
Positive IP detected in | human placenta tissue |
Positive IHC detected in | human lung cancer tissue, human liver tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human lung cancer tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:10000-1:40000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 35 publications below |
IHC | See 62 publications below |
IF | See 94 publications below |
FC | See 5 publications below |
Product Information
60143-1-Ig targets CD206 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, pig |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | mannose receptor, C type 1 |
Calculated Molecular Weight | 166 kDa |
Observed Molecular Weight | 170 kDa |
GenBank Accession Number | NM_002438 |
Gene Symbol | CD206 |
Gene ID (NCBI) | 4360 |
RRID | AB_2144924 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD206, also named as MMR, CLEC13D and MRC1, is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. CD206 has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment. CD206 is a 170 kDa transmembrane protein which contains 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. It is expressed on most tissue macrophages, in vitro derived dendritic cells, lymphatic and sinusoidal endothelia. This antibody recognizes the intracellular carboxy-terminal part of CD206 and MRC1L1.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for CD206 antibody 60143-1-Ig | Download protocol |
IHC protocol for CD206 antibody 60143-1-Ig | Download protocol |
IF protocol for CD206 antibody 60143-1-Ig | Download protocol |
IP protocol for CD206 antibody 60143-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Neurosci A phenotypic screening platform for identifying chemical modulators of astrocyte reactivity | ||
Bioact Mater Profibrogenic macrophage-targeted delivery of mitochondrial protector via exosome formula for alleviating pulmonary fibrosis | ||
ACS Nano Tailoring Chemoimmunostimulant Bioscaffolds for Inhibiting Tumor Growth and Metastasis after Incomplete Microwave Ablation. | ||
ACS Nano Smart Liposomal Nanocarrier Enhanced the Treatment of Ischemic Stroke through Neutrophil Extracellular Traps and Cyclic Guanosine Monophosphate-Adenosine Monophosphate Synthase-Stimulator of Interferon Genes (cGAS-STING) Pathway Inhibition of Ischemic Penumbra | ||
Cancer Commun (Lond) Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer | ||
Adv Sci (Weinh) Nanocomposite Hydrogel for Real-Time Wound Status Monitoring and Comprehensive Treatment |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Kenzo (Verified Customer) (03-07-2023) | Works great with minimal background for immunofluorescence
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