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CD206 Antibody 6 Publications

Mouse Monoclonal| Catalog number: 60143-1-Ig |CloneNo.: 2A6A10

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Con: 150 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
Application not tested? Please see our Guarantee
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.


Images See all

CD206 Antibody WB human placenta tissue 60143-1-Ig

human placenta tissue were subjected to SDS PAGE followed by western blot with 60143-1-Ig( CD206 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours

CD206 Antibody IHC human lung cancer tissue 60143-1-Ig

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60143-1-Ig (CD206 antibody) at dilution of 1:20000 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

CD206 Antibody IHC human lung cancer tissue 60143-1-Ig

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60143-1-Ig (CD206 antibody) at dilution of 1:20000 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

CD206 Antibody IP human placenta tissue 60143-1-Ig

IP Result of anti-CD206 (IP:60143-1-Ig, 5ug; Detection:60143-1-Ig 1:300) with human placenta tissue lysate 1520ug.

CD206 Antibody FC RAW 264.7 cells 60143-1-Ig

1X10^6 RAW 264.7 cells were stained with 0.2ug CD206 antibody (60143-1-Ig, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500.

Species specificity:
human, mouse, rat
Positive WB detected in:
human placenta tissue, human liver tissue
Positive IP detected in:
human placenta tissue
Positive IHC detected in:
human lung cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected in:
RAW 264.7 cells
Recommended dilution:
WB : 1:1000-1:4000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:10000-1:40000
Sample-dependent, check data in Validation tab

Product Information

Source:
Mouse
Purification method:
Caprylic acid/ammonium sulfate precipitation
Isotype:
IgG2a
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
mannose receptor, C type 1
Calculated molecular weight:
166 kDa
Observed molecular weight:
170 kDa
GenBank accession number:
NM_002438
Gene ID (NCBI):
4360
Gene symbol
MRC1
Synonyms
CD206, CLEC13D, Macrophage mannose receptor 1, Mannose receptor, mannose receptor, C type 1, MMR, MRC1

Background

CD206, also named as MMR, CLEC13D and MRC1, is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. CD206 has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment. CD206 is a 170 kDa transmembrane protein which contains 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. It is expressed on most tissue macrophages, in vitro derived dendritic cells, lymphatic and sinusoidal endothelia. This antibody recognizes the intracellular carboxy-terminal part of CD206 and MRC1L1.


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