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  • KD/KO Validated

CD206 Monoclonal antibody

CD206 Monoclonal Antibody for FC, IHC, IP, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

CloneNo.

2A6A10

Cat no : 60143-1-Ig

Synonyms

CD206, CLEC13D, Macrophage mannose receptor 1, Mannose receptor, mannose receptor, C type 1, MMR, MRC1



Tested Applications

Positive WB detected inhuman placenta tissue, human liver tissue
Positive IP detected inhuman placenta tissue
Positive IHC detected inhuman lung cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inRAW 264.7 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:10000-1:40000
Sample-dependent, check data in validation data gallery

Product Information

60143-1-Ig targets CD206 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name mannose receptor, C type 1
Calculated molecular weight 166 kDa
Observed molecular weight 170 kDa
GenBank accession numberNM_002438
Gene symbol MRC1
Gene ID (NCBI) 4360
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD206, also named as MMR, CLEC13D and MRC1, is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. CD206 has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment. CD206 is a 170 kDa transmembrane protein which contains 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. It is expressed on most tissue macrophages, in vitro derived dendritic cells, lymphatic and sinusoidal endothelia. This antibody recognizes the intracellular carboxy-terminal part of CD206 and MRC1L1.

Protocols

Product Specific Protocols
WB protocol for CD206 antibody 60143-1-IgDownload protocol
IHC protocol for CD206 antibody 60143-1-IgDownload protocol
IP protocol for CD206 antibody 60143-1-IgDownload protocol
FC protocol for CD206 antibody 60143-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Int J Mol Sci

Trichostatin A Alleviates Renal Interstitial Fibrosis Through Modulation of the M2 Macrophage Subpopulation.

Authors - Wei-Cheng Tseng
ratFC

In Vitro Cell Dev Biol Anim

Effect of CSE on M1/M2 polarization in alveolar and peritoneal macrophages at different concentrations and exposure in vitro.

Authors - Haoshen Feng
mouseIF

Biomed Mater

The observed difference of RAW264.7 macrophage phenotype on mineralized collagen and hydroxyapatite.

Authors - Xiudi Shi
ratIHC

Brain Res

Hydrogen inhalation inhibits microglia activation and neuroinflammation in a rat model of traumatic brain injury.

Authors - Qing-Hui Zhao
ratIF

Physiol Behav

Non-neuronal crosstalk promotes an inflammatory response in nodose ganglia cultures after exposure to byproducts from gram positive, high-fat-diet-associated gut bacteria.

Authors - Carolina R Cawthon
mouseWB

Exp Cell Res

Involvement of JNK signaling in IL4-induced M2 macrophage polarization.

Authors - Jin Hao
  • KD Validated