- Featured Product
- KD/KO Validated
CD206 Monoclonal antibody
CD206 Monoclonal Antibody for FC, IHC, IP, WB, ELISA
Cat no : 60143-1-Ig
|Positive WB detected in||human placenta tissue, human liver tissue|
|Positive IP detected in||human placenta tissue|
|Positive IHC detected in||human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||RAW 264.7 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:10000-1:40000|
|Sample-dependent, check data in validation data gallery|
60143-1-Ig targets CD206 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||mouse, rat|
|Host / Isotype||Mouse / IgG2a|
|Full Name||mannose receptor, C type 1|
|Calculated molecular weight||166 kDa|
|Observed molecular weight||170 kDa|
|GenBank accession number||NM_002438|
|Gene ID (NCBI)||4360|
|Purification Method||Caprylic acid/ammonium sulfate precipitation|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CD206, also named as MMR, CLEC13D and MRC1, is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. CD206 has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment. CD206 is a 170 kDa transmembrane protein which contains 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. It is expressed on most tissue macrophages, in vitro derived dendritic cells, lymphatic and sinusoidal endothelia. This antibody recognizes the intracellular carboxy-terminal part of CD206 and MRC1L1.
|Product Specific Protocols|
|WB protocol for CD206 antibody 60143-1-Ig||Download protocol|
|IHC protocol for CD206 antibody 60143-1-Ig||Download protocol|
|IP protocol for CD206 antibody 60143-1-Ig||Download protocol|
|FC protocol for CD206 antibody 60143-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
In Vitro Cell Dev Biol Anim
Effect of CSE on M1/M2 polarization in alveolar and peritoneal macrophages at different concentrations and exposure in vitro.
The observed difference of RAW264.7 macrophage phenotype on mineralized collagen and hydroxyapatite.
Exp Cell Res
Involvement of JNK signaling in IL4-induced M2 macrophage polarization.
Appl Microbiol Biotechnol
Mannose-modified chitosan microspheres enhance OprF-OprI-mediated protection of mice against Pseudomonas aeruginosa infection via induction of mucosal immunity.
Clin Sci (Lond)
Particulate matter exposure aggravates osteoarthritis severity.