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  • KD/KO Validated

CD206 Monoclonal antibody

CD206 Monoclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2A6A10

Cat no : 60143-1-Ig

Synonyms

CD206, CLEC13D, Macrophage mannose receptor 1, Mannose receptor, mannose receptor, C type 1, MMR, MRC1



Tested Applications

Positive WB detected inhuman placenta tissue, human liver tissue
Positive IP detected inhuman placenta tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inRAW 264.7 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:10000-1:40000
Sample-dependent, check data in validation data gallery

Product Information

60143-1-Ig targets CD206 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name mannose receptor, C type 1
Calculated molecular weight 166 kDa
Observed molecular weight 170 kDa
GenBank accession numberNM_002438
Gene symbol MRC1
Gene ID (NCBI) 4360
Conjugate Unconjugated
Form Liquid
Purification Method Caprylic acid/ammonium sulfate precipitation
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD206, also named as MMR, CLEC13D and MRC1, is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. CD206 has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment. CD206 is a 170 kDa transmembrane protein which contains 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. It is expressed on most tissue macrophages, in vitro derived dendritic cells, lymphatic and sinusoidal endothelia. This antibody recognizes the intracellular carboxy-terminal part of CD206 and MRC1L1.

Protocols

Product Specific Protocols
WB protocol for CD206 antibody 60143-1-IgDownload protocol
IHC protocol for CD206 antibody 60143-1-IgDownload protocol
IP protocol for CD206 antibody 60143-1-IgDownload protocol
FC protocol for CD206 antibody 60143-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratFC

In Vitro Cell Dev Biol Anim

Effect of CSE on M1/M2 polarization in alveolar and peritoneal macrophages at different concentrations and exposure in vitro.

Authors - Haoshen Feng
mouseIF

Biomed Mater

The observed difference of RAW264.7 macrophage phenotype on mineralized collagen and hydroxyapatite.

Authors - Xiudi Shi
mouseWB

Exp Cell Res

Involvement of JNK signaling in IL4-induced M2 macrophage polarization.

Authors - Jin Hao
  • KD Validated
rat

Appl Microbiol Biotechnol

Mannose-modified chitosan microspheres enhance OprF-OprI-mediated protection of mice against Pseudomonas aeruginosa infection via induction of mucosal immunity.

Authors - Ziyin Cui
ratWB,IHC

Clin Sci (Lond)

Particulate matter exposure aggravates osteoarthritis severity.

Authors - Kuo-Ti Peng
mouseIF

Theranostics

PMID: 31903147

Authors - Xiongfa Ji