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- KD/KO Validated
CD206 Monoclonal antibody
CD206 Monoclonal Antibody for FC, IHC, IP, WB,ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse and More (1)
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
2A6A10
Cat no : 60143-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human placenta tissue, human liver tissue |
| Positive IP detected in | human placenta tissue |
| Positive IHC detected in | human lung cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | RAW 264.7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:10000-1:40000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 4 publications below |
| IHC | See 9 publications below |
| IF | See 10 publications below |
| FC | See 3 publications below |
Product Information
60143-1-Ig targets CD206 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | mannose receptor, C type 1 |
| Calculated molecular weight | 166 kDa |
| Observed molecular weight | 170 kDa |
| GenBank accession number | NM_002438 |
| Gene symbol | MRC1 |
| Gene ID (NCBI) | 4360 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD206, also named as MMR, CLEC13D and MRC1, is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. CD206 has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment. CD206 is a 170 kDa transmembrane protein which contains 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. It is expressed on most tissue macrophages, in vitro derived dendritic cells, lymphatic and sinusoidal endothelia. This antibody recognizes the intracellular carboxy-terminal part of CD206 and MRC1L1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD206 antibody 60143-1-Ig | Download protocol |
| IHC protocol for CD206 antibody 60143-1-Ig | Download protocol |
| IP protocol for CD206 antibody 60143-1-Ig | Download protocol |
| FC protocol for CD206 antibody 60143-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Mol Sci Trichostatin A Alleviates Renal Interstitial Fibrosis Through Modulation of the M2 Macrophage Subpopulation. | ||
Acta Biomater Soft apoptotic-cell-inspired nanoparticles persistently bind to macrophage membranes and promote anti-inflammatory and pro-healing effects. | ||
In Vitro Cell Dev Biol Anim Effect of CSE on M1/M2 polarization in alveolar and peritoneal macrophages at different concentrations and exposure in vitro. | ||
Biomed Mater The observed difference of RAW264.7 macrophage phenotype on mineralized collagen and hydroxyapatite. | ||
Brain Res Hydrogen inhalation inhibits microglia activation and neuroinflammation in a rat model of traumatic brain injury. | ||
Physiol Behav Non-neuronal crosstalk promotes an inflammatory response in nodose ganglia cultures after exposure to byproducts from gram positive, high-fat-diet-associated gut bacteria. |
