CoraLite® Plus 488-conjugated CD206 Monoclonal antibody

CD206 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Publications

4

CloneNo.

2A6A10

Cat no : CL488-60143

Synonyms

CD206, CLEC13D, Macrophage mannose receptor 1, mannose receptor, C type 1, MMR, MRC1

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Validation Data Gallery

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  IF Staining of human lung cancer using CL488-60143

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 488 CD206 antibody (CL488-60143, Clone: 2A6A10 ) at dilution of 1:200.

• 

  IF Staining of human lung cancer using CL488-60143

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 488 CD206 antibody (CL488-60143, Clone: 2A6A10 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: human lung cancer tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 4 publications below

Product Information

CL488-60143 targets CD206 in IF applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: mannose receptor, C type 1
• Calculated Molecular Weight: 166 kDa
• GenBank Accession Number: NM_002438
• Gene Symbol: MRC1
• Gene ID (NCBI): 4360
• RRID: AB_2883105
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD206, also named as MMR, CLEC13D and MRC1, is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. CD206 has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment. CD206 is a 170 kDa transmembrane protein which contains 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. It is expressed on most tissue macrophages, in vitro derived dendritic cells, lymphatic and sinusoidal endothelia. This antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Publications

Species	Application	Title
	IF	Acta Biochim Biophys Sin (Shanghai); 5-HMF attenuates inflammation and demyelination in experimental autoimmune encephalomyelitis mice by inhibiting the MIF-CD74 interaction; Authors - Dongsheng Guan
human	IF	Cancer Gene Ther; CDK5RAP3 acts as a tumour suppressor in gastric cancer through the infiltration and polarization of tumour-associated macrophages; Authors - Jia-Bin Wang
	IF	J Ethnopharmacol; Elsholtzia bodinieri Vaniot ameliorated acute lung injury in mice by regulating pyroptosis, inflammation, oxidative stress and macrophage polarization; Authors - Xiaoqian Jiang
	IF	Phytomedicine; Proteomics and transcriptomics explore the effect of mixture of herbal extract on diabetic wound healing process; Authors - Yang Liu