MRC2 Polyclonal antibody

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• MRC2 Polyclonal antibody (21410-1-AP)

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  WB analysis using 21410-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21410-1-AP (MRC2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

MRC2 Polyclonal Antibody for WB, ELISA

Cat No. 21410-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

ENDO180, C-type mannose receptor 2, C-type lectin domain family 13 member E, CLEC13E, CD280

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Tested Applications

• Positive WB detected in: HeLa cells, Saos-2 cells, SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21410-1-AP targets MRC2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15570

  Product name: Recombinant human MRC2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1066-1415 aa of BC146647

  Sequence: PSPAPSGNKPTSCAVVLHSPSAHFTGRWDDRSCTEETHGFICQKGTDPSLSPSPAALPPAPGTELSYLNGTFRLLQKPLRWHDALLLCESHNASLAYVPDPYTQAFLTQAARGLRTPLWIGLAGEEGSRRYSWVSEEPLNYVGWQDGEPQQPGGCTYVDVDGAWRTTSCDTKLQGAVCGVSSGPPPPRRISYHGSCPQGLADSAWIPFREHCYSFHMELLLGHKEARQRCQRAGGAVLSILDEMENVFVWEHLQSYEGQSRGAWLGMNFNPKGGTLVWQDNTAVNYSNWGPPGLGPSMLSHNSCYWIQSNSGLWRPGACTNITMGVVCKLPRAEQSSFSPSALPENPAAL

• Full Name: mannose receptor, C type 2
• Calculated Molecular Weight: 1479 aa, 167 kDa
• Observed Molecular Weight: 170 kDa
• GenBank Accession Number: BC146647
• Gene Symbol: MRC2
• Gene ID (NCBI): 9902
• RRID: AB_3669367
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UBG0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C-type mannose receptor 2 (MRC2), also known as CD280, CLEC13E, ENDO180, KIAA0709, or UPARAP, is a type I transmembrane glycoprotein that belongs to the macrophage mannose receptor protein family (PMID: 10683150; 10636902; 9734811). It contains an N-terminal cysteine-rich domain, a fibronectin type II domain (FNII), eight C-type-lectin-like domains, and a transmembrane region followed by a small cytoplasmic domain (PMID: 34012764). MRC2 is a recycling endocytic receptor that functions in both these cellular activities by promoting cell migration and uptake of collagens for intracellular degradation (PMID: 17974964). MRC2 is expressed in several cells and organs including fibroblasts, endothelial cells, and macrophages. Altered MRC2 expression has been associated with the development and progression of different cancers, including head and neck squamous cell carcinoma, breast cancer, hepatocellular cancer, and prostate cancer (PMID: 36188226; 37666484; 17974964; 25162823).