MRFAP1 Polyclonal antibody

MRFAP1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 11639-1-AP

Synonyms

MRFAP1, PAM14, PGR1, Protein PGR1

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Validation Data Gallery

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  WB analysis of Jurkat using 11639-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11639-1-AP (MRFAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 11639-1-AP

  IP result of anti-MRFAP1 (IP:11639-1-AP, 4ug; Detection:11639-1-AP 1:300) with Jurkat cells lysate 1400 ug.

• 

  IHC staining of human gliomas using 11639-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11639-1-AP (MRFAP1 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

11639-1-AP targets MRFAP1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MRFAP1 fusion protein Ag2227
• Full Name: Mof4 family associated protein 1
• Calculated Molecular Weight: 127 aa, 15 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC022797
• Gene Symbol: MRFAP1
• Gene ID (NCBI): 93621
• RRID: AB_2145271
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MRFAP1, also named as PAM14 and PGR1, is a 14 kDa protein which associated with MRG. It is found in a complex composed of MORF4L1, MRFAP1 and RB1. MRFAP1 interacts via its N-terminus with MORF4L1. And it interacts with CSTB and MORF4L2.

Publications

Species	Application	Title
human	WB	Mol Cell Proteomics; Characterisation of Native Protein Complexes and Protein Isoform Variation using Size-Fractionation Based Quantitative Proteomics.; Authors - Kirkwood Kathryn J KJ
human	WB	Mol Cell Proteomics; Quantitative proteomic analysis of cellular protein modulation upon inhibition of the NEDD8-activating enzyme by MLN4924.; Authors - Hua Liao
human,monkey	WB,IP	J Cell Biochem; Interaction of Sedlin with PAM14.; Authors - Liu Xiaoying X
human	IF, WB	Oncotarget; FBXW8-dependent degradation of MRFAP1 in anaphase controls mitotic cell death.; Authors - Duan-Zhuo Li

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