MRI1 Polyclonal antibody, PBS Only

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  WB analysis of HT-1080 using 19916-1-AP (same clone as 19916-1-PBS)

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 19916-1-AP (MRI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19916-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HL-60 using 19916-1-AP (same clone as 19916-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 19916-1-AP (MRI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19916-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat kidney using 19916-1-AP (same clone as 19916-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 19916-1-AP (MRI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19916-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat kidney using 19916-1-AP (same clone as 19916-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 19916-1-AP (MRI1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19916-1-PBS in a different storage buffer formulation.

MRI1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 19916-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

19916-1-PBS targets MRI1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13809

  Product name: Recombinant human MRI1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-369 aa of BC001703

  Sequence: MTLEAIRYSRGSLQILDQLLLPKQSRYEAVGSVHQAWEAIRAMKVRGAPAIALVGCLSLAVELQAGAGGPGLAALVAFVRDKLSFLVTARPTAVNMARAARDLADVAAREAEREGATEEAVRERVICCTEDMLEKDLRDNRSIGDLGARHLLERVAPSGGKVTVLTHCNTGALATAGYGTALGVIRSLHSLGRLEHAFCTETRPYNQGARLTAFELVYEQIPATLITDSMVAAAMAHRGVSAVVVGADRVVANGDTANKVGTYQLAIVAKHHGIPFYVAAPSSSCDLRLETGKEIIIEERPGQELTDVNGVRIAAPGIGVWNPAFDVTPHDLITGGIITELGVFAPEELRTALTTTISSRDGTLDGPQM

• Full Name: methylthioribose-1-phosphate isomerase homolog (S. cerevisiae)
• Calculated Molecular Weight: 369 aa, 39 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC001703
• Gene Symbol: MRI1
• Gene ID (NCBI): 84245
• RRID: AB_10638924
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BV20
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MRI1 is also named as MRDI and belongs to the eIF-2B alpha/beta/delta subunits family. MRI1 promotes cell invasion in response to constitutive RhoA activation by promoting FAK tyrosine phosphorylation (PMID:19620624). MRDI acts as a methylthioribose-1-phosphate (MTR-1-P) isomerase, catalyzing a critical step in methionine salvage. Second, MRDI promotes and is necessary for melanoma cell invasion, independent of its catalytic activity (PMID:23859498).