MRP2 Polyclonal antibody

MRP2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24893-1-AP

Synonyms

ABC30, ABCC2, MRP2



Tested Applications

Positive WB detected inHepG2 cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24893-1-AP is MRP2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRP2 fusion protein Ag18694
Full Name ATP-binding cassette, sub-family C (CFTR/MRP), member 2
Calculated molecular weight 1545 aa, 174 kDa
Observed molecular weight 190-250 kDa
GenBank accession numberBC136419
Gene symbol ABCC2
Gene ID (NCBI) 1244
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Multi-drug resistance protein 2 (MRP2), also known as ABCC2, is an ATP binding cassette (ABC) transporter responsible for biliary excretion of xenobiotics, endobiotics, and their metabolites. Deficiency in ABCC2 results in the clinical disorder Dubin-Johnson syndrome. MRP2 is found to be expressed in a variety of human cancers, and is associated with resistance of tumor cells to various anticancer drugs including cisplatin. The predicted molecular weight of MRP2 is 174 kDa, while mature MRP2 usually has a slower migration around 190-250 kDa due to the glycosylation. (16434545, 18834541, 23045960)

Protocols

Product Specific Protocols
WB protocol for MRP2 antibody 24893-1-APDownload protocol
IHC protocol for MRP2 antibody 24893-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Molecules

Effect of Stereochemical Configuration on the Transport and Metabolism of Catechins from Green Tea across Caco-2 Monolayers.

Authors - Zeyi Ai
humanWB

Biochem Biophys Res Commun

Formononetin ameliorates cholestasis by regulating hepatic SIRT1 and PPARα.

Authors - Shu Yang
mouseWB

ACS Nano

Variability in the Clearance of Lead Oxide Nanoparticles Is Associated with Alteration of Specific Membrane Transporters.

Authors - Jana Dumková
humanWB

Neoplasma

Knockdown of USP9X reverses cisplatin resistance by decreasing β-catenin expression in nasopharyngeal carcinoma cells.

Authors - Wen-Xuan Lu
humanWB

Oncol Lett

Effects of histone deacetylase inhibitors on ATP-binding cassette transporters in lung cancer A549 and colorectal cancer HCT116 cells.

Authors - Hao Wang
humanWB

J Virol Methods

Revival, characterization, and hepatitis B virus infection of cryopreserved human fetal hepatocytes.

Authors - Ming Zhou

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

SHENG-FU (Verified Customer) (01-15-2018)

there were multiple bands on the membrane, and the band under 130 kDa were a high expression. But around 190 kDa -250 kDa there are a single band which might be the correct one.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Rat primary astrocyte
MRP2 Antibody Western Blot, validation (1:500 dilution) in Rat primary astrocyte (Cat no:24893-1-AP)