|Positive WB detected in||HepG2 cells|
|Positive IHC detected in||human small intestine tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 24893-1-AP is MRP2 Fusion Protein expressed in E. coli.
|Cited Reactivity||human, monkey|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MRP2 fusion protein Ag18694|
|Full Name||ATP-binding cassette, sub-family C (CFTR/MRP), member 2|
|Calculated molecular weight||1545 aa, 174 kDa|
|Observed molecular weight||190-250 kDa|
|GenBank accession number||BC136419|
|Gene ID (NCBI)||1244|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Multi-drug resistance protein 2 (MRP2), also known as ABCC2, is an ATP binding cassette (ABC) transporter responsible for biliary excretion of xenobiotics, endobiotics, and their metabolites. Deficiency in ABCC2 results in the clinical disorder Dubin-Johnson syndrome. MRP2 is found to be expressed in a variety of human cancers, and is associated with resistance of tumor cells to various anticancer drugs including cisplatin. The predicted molecular weight of MRP2 is 174 kDa, while mature MRP2 usually has a slower migration around 190-250 kDa due to the glycosylation. (16434545, 18834541, 23045960)
Effect of Stereochemical Configuration on the Transport and Metabolism of Catechins from Green Tea across Caco-2 Monolayers.
Biochem Biophys Res Commun
Formononetin ameliorates cholestasis by regulating hepatic SIRT1 and PPARα.
Variability in the Clearance of Lead Oxide Nanoparticles Is Associated with Alteration of Specific Membrane Transporters.
Effects of histone deacetylase inhibitors on ATP-binding cassette transporters in lung cancer A549 and colorectal cancer HCT116 cells.
J Virol Methods
Revival, characterization, and hepatitis B virus infection of cryopreserved human fetal hepatocytes.
Comparison of Pregnane X Receptor Antagonists for Enhancing the Antitumor Effect of Cisplatin.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
SHENG-FU (Verified Customer) (01-15-2018)
there were multiple bands on the membrane, and the band under 130 kDa were a high expression. But around 190 kDa -250 kDa there are a single band which might be the correct one.