|Positive WB detected in
|Positive IHC detected in
|human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)
|WB : 1:500-1:1000
|IHC : 1:20-1:200
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
The immunogen of 24893-1-AP is MRP2 Fusion Protein expressed in E. coli.
|human, rat, monkey
|Host / Isotype
|Rabbit / IgG
|MRP2 fusion protein Ag18694
|ATP-binding cassette, sub-family C (CFTR/MRP), member 2
|Calculated molecular weight
|1545 aa, 174 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Multi-drug resistance protein 2 (MRP2), also known as ABCC2, is an ATP binding cassette (ABC) transporter responsible for biliary excretion of xenobiotics, endobiotics, and their metabolites. Deficiency in ABCC2 results in the clinical disorder Dubin-Johnson syndrome. MRP2 is found to be expressed in a variety of human cancers, and is associated with resistance of tumor cells to various anticancer drugs including cisplatin. The predicted molecular weight of MRP2 is 174 kDa, while mature MRP2 usually has a slower migration around 190-250 kDa due to the glycosylation. (16434545, 18834541, 23045960)
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
SHENG-FU (Verified Customer) (01-15-2018)
there were multiple bands on the membrane, and the band under 130 kDa were a high expression. But around 190 kDa -250 kDa there are a single band which might be the correct one.