ABCC5 Polyclonal antibody

ABCC5 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29792-1-AP

Synonyms

ABC33, ABCC5, DKFZp686C1782, EST277145, MOAT C, MOATC, MRP5, pABC11, SMRP

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Validation Data Gallery

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  WB analysis using 29792-1-AP

  Boiled and unboiled MCF-7 lysates were subjected to SDS PAGE followed by western blot with 29792-1-AP (ABCC5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 29792-1-AP

  Unboiled HepG2 cells and unboiled MCF-7 cells lysates were subjected to SDS PAGE followed by western blot with 29792-1-AP (ABCC5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: MCF-7 cells, unboiled HepG2 cells, unboiled MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29792-1-AP targets ABCC5 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ABCC5 fusion protein Ag31680
• Full Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 5
• Calculated Molecular Weight: 161 kDa
• Observed Molecular Weight: 161-200 kDa
• GenBank Accession Number: NM_005688
• Gene Symbol: ABCC5
• Gene ID (NCBI): 10057
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ABCC5, also named as MOAT-C, pABC11, SMRP and MRP5, belongs to the ABC transporter superfamily, ABCC family and Conjugate transporter (TC 3.A.1.208) subfamily. ABCC5 acts as a multispecific organic anion pump which can transport nucleotide analogs. ABCC5 functions in the cellular export of its substrate, cyclic nucleotides. This export contributes to the degradation of phosphodiesterases and possibly an elimination pathway for cyclic nucleotides. Studies show that ABCC5 provides resistance to thiopurine anticancer drugs, 6-mercatopurine and thioguanine, and the anti-HIV drug 9-(2-phosphonylmethoxyethyl) adenine. ABCC5 may be involved in resistance to thiopurines in acute lymphoblastic leukemia and antiretroviral nucleoside analogs in HIV-infected patients. Alternative splicing of this gene has been detected; however, the complete sequence and translation initiation site is unclear. Since it is glycosylated, the apparent molecular weight of ABCC5 could be variable, ranging from 161 kDa to 200 kDa (PMID: 10893247; PMID: 15897250; PMID: 31338999).