Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, HEK-293 mitochondrial |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human testis tissue, mouse kidney tissue, human placenta tissue, mouse adrenal gland tissue, human thyroid cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16652-1-AP targets MRPL10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10087 Product name: Recombinant human MRPL10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-261 aa of BC052601 Sequence: MAAAVAGMLRGGLLPQAGRLPTLQTVRYGSKAVTRHRRVMHFQRQKLMAVTEYIPPKPAIHPSCLPSPPSPPQEEIGLIRLLRREIAAVFQDNRMIAVCQNVALSAEDKLLMRHQLRKHKILMKVFPNQVLKPFLEDSKYQNLLPLFVGHNMLLVSEEPKVKEMVRILRTVPFLPLLGGCIDDTILSRQGFINYSKLPSLPLVQGELVGGLTCLTAQTHSLLQHQPLQLTTLLDQYIREQREKDSVMSANGKPDPDTVPDS Predict reactive species |
| Full Name | mitochondrial ribosomal protein L10 |
| Calculated Molecular Weight | 261 aa, 29 kDa |
| Observed Molecular Weight | 29 kDa |
| GenBank Accession Number | BC052601 |
| Gene Symbol | MRPL10 |
| Gene ID (NCBI) | 124995 |
| RRID | AB_2282109 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7Z7H8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MRPL10 antibody 16652-1-AP | Download protocol |
| IHC protocol for MRPL10 antibody 16652-1-AP | Download protocol |
| IP protocol for MRPL10 antibody 16652-1-AP | Download protocol |
| WB protocol for MRPL10 antibody 16652-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MRPL10 Polyclonal antibody (Cat# 16652-1-AP) has 3 citations, all in Western Blot applications using human samples. Citations appear in Cell (mitochondrial gene expression silencing), Nature Structural & Molecular Biology (ribosome assembly in human mitochondria), and EMBO Reports (mitochondrial calcium uniporter complex and T-cell immune responses). The research fields span mitochondrial biology, ribosome biogenesis, and mitochondrial calcium signaling in immunity.
| Species | Application | Title |
|---|---|---|
EMBO Rep Mitochondrial calcium uniporter complex controls T-cell-mediated immune responses |
Reviews
What customers say
The product received a 5-star rating from a user who applied it in immunofluorescence. The reviewer described it as "good for imaging," indicating satisfactory performance for fluorescence-based applications. Overall, the feedback is positive, with no reported issues regarding specificity, background, or ease of use.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Boyan (Verified Customer) (01-31-2025) | good for imaging
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