Tested Applications
| Positive WB detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15040-1-AP targets MRPL14 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6808 Product name: Recombinant human MRPL14 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-145 aa of BC065005 Sequence: MAFFTGLWGPFTCVSRVLSHHCFSTTGSLSAIQKMTRVRVVDNSALGNSPYHRAPRCIHVYKKNGVGKVGDQILLAIKGQKKKALIVGHCMPGPRMTPRFDSNNVVLIEDNGNPVGTRIKTPIPTSLRKREGEYSKVLAIAQNFV Predict reactive species |
| Full Name | mitochondrial ribosomal protein L14 |
| Calculated Molecular Weight | 16 kDa |
| Observed Molecular Weight | 16 kDa |
| GenBank Accession Number | BC065005 |
| Gene Symbol | MRPL14 |
| Gene ID (NCBI) | 64928 |
| RRID | AB_2878102 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6P1L8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MRPL14 antibody 15040-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MRPL14 Polyclonal antibody (Cat# 15040-1-AP) has 4 total citations. These appear in Signal Transduction and Targeted Therapy, Nature Structural & Molecular Biology, EMBO Molecular Medicine, and Medicine (Baltimore). The cited studies focus on mitochondrial biology, covering topics such as oxidative phosphorylation in podocytes, mitochondrial ribosome assembly in humans, mitochondrial lipid homeostasis and cristae maintenance, and the causal role of mitochondrial proteins in aortic aneurysms.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Fructose directly remodels the translocase of the outer membrane to impair oxidative phosphorylation in podocytes | ||
EMBO Mol Med SLC25A46 is required for mitochondrial lipid homeostasis and cristae maintenance and is responsible for Leigh syndrome. | ||
Medicine (Baltimore) Causal role of mitochondrial proteins in aortic aneurysms: Evidence from Mendelian randomization, transcriptomic analysis, and experimental validation |

