MRPL19 Recombinant monoclonal antibody

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  WB analysis using 87422-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87422-1-RR (MRPL19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity of 87422-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87422-1-RR against Human MRPL19 were performed. The affinity constant is 1.43 nM.

MRPL19 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87422-1-RR

Clone No.252836E9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

KIAA0104, L15mt, L19mt, Large ribosomal subunit protein bL19m, MRP L15

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, ACHN cells, A549 cells, SK-N-SH cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87422-1-RR targets MRPL19 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag9738

  Product name: Recombinant human MRPL19 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-292 aa of BC030144

  Sequence: MAACIAAGHWAAMGLGRSFQAARTLLPPPASIACRVHAGPVRQQSTGPSEPGAFQPPPKPVIVDKHRPVEPERRFLSPEFIPRRGRTDPLKFQIERKDMLERRKVLHIPEFYVGSILRVTTADPYASGKISQFLGICIQRSGRGLGATFILRNVIEGQGVEICFELYNPRVQEIQVVKLEKRLDDSLLYLRDALPEYSTFDVNMKPVVQEPNQKVPVNELKVKMKPKPWSKRWERPNFNIKGIRFDLCLTEQQMKEAQKWNQPWLEFDMMREYDTSKIEAAIWKEIEASKRS

• Full Name: mitochondrial ribosomal protein L19
• Calculated Molecular Weight: 292 aa, 34 kDa
• Observed Molecular Weight: 30-35 kDa
• GenBank Accession Number: BC030144
• Gene Symbol: MRPL19
• Gene ID (NCBI): 9801
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P49406
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.