MRPL2 Polyclonal antibody

• 

  WB analysis using 16492-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16492-1-AP (MRPL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

MRPL2 Polyclonal Antibody for WB, ELISA

Cat No. 16492-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

CGI 22, L2mt, MRP L14, MRP L2, MRPL2, RPML14

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

16492-1-AP targets MRPL2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9618

  Product name: Recombinant human MRPL2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-305 aa of BC020212

  Sequence: MALCALTRALRSLNLAPPTVAAPAPSLFPAAQMMNNGLLQQPSALMLLPCRPVLTSVALNANFVSWKSRTKYTITPVKMRKSGGRDHTGRIRVHGIGGGHKQRYRMIDFLRFRPEETKSGPFEEKVIQVRYDPCRSADIALVAGGSRKRWIIATENMQAGDTILNSNHIGRMAVAAREGDAHPLGALPVGTLINNVESEPGRGAQYIRAAGTCGVLLRKVNGTAIIQLPSKRQMQVLETCVATVGRVSNVDHNKRVIGKAGRNRWLGKRPNSGRWHRKGGWAGRKIRPLPPMKSYVKLPSASAQS

• Full Name: mitochondrial ribosomal protein L2
• Calculated Molecular Weight: 305 aa, 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC020212
• Gene Symbol: MRPL2
• Gene ID (NCBI): 51069
• RRID: AB_2145746
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5T653
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Mol Life Sci; GTPBP8 is required for mitoribosomal biogenesis and mitochondrial translation; Authors - Liang Wang
mouse	WB	iScience; Proteostasis regulated by testis-specific ribosomal protein RPL39L maintains mouse spermatogenesis.; Authors - Qianxing Zou
human	WB	Mol Cell; Microprotein SMIM26 drives oxidative metabolism via serine-responsive mitochondrial translation; Authors - Jiemin Nah