MRPL24 Polyclonal antibody

• Featured Product
• KD/KO Validated

MRPL24 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 16224-1-AP

Synonyms

MRP-L24, MRP L24, MRP L18, Large ribosomal subunit protein uL24m, L24mt

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Validation Data Gallery

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  WB analysis of HEK-293 using 16224-1-AP

  WB result of MRPL24 antibody (16224-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPL24 transfected HEK-293 cells.

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  WB analysis using 16224-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16224-1-AP (MRPL24 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16224-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16224-1-AP (MRPL24 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 16224-1-AP

  IP result of anti-MRPL24 (IP:16224-1-AP, 4ug; Detection:16224-1-AP 1:1000) with HEK-293 cells lysate 1480ug.

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Tested Applications

• Positive WB detected in: mouse liver tissue, HEK-293 cells
• Positive IP detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

16224-1-AP targets MRPL24 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MRPL24 fusion protein Ag9277
• Full Name: mitochondrial ribosomal protein L24
• Calculated Molecular Weight: 216 aa, 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC016700
• Gene Symbol: MRPL24
• Gene ID (NCBI): 79590
• RRID: AB_2297885
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	J Cell Biol; Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness.; Authors - Uwe Richter
human	WB	Cell Mol Life Sci; GTPBP8 is required for mitoribosomal biogenesis and mitochondrial translation; Authors - Liang Wang
	WB	J Biol Chem; The Pseudouridine Synthase RPUSD4 is an essential component of Mitochondrial RNA Granules.; Authors - Sofia Zaganelli
human	WB	Proteomes; Utilizing Optimized Tools to Investigate PTM Crosstalk: Identifying Potential PTM Crosstalk of Acetylated Mitochondrial Proteins.; Authors - Henrick Horita