MRPL27 Polyclonal antibody

MRPL27 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14765-1-AP

Synonyms

L27mt, MRP L27, MRPL27

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Validation Data Gallery

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  WB analysis of A431 using 14765-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 14765-1-AP (MRPL27 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 14765-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14765-1-AP (MRPL27 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 14765-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 14765-1-AP (MRPL27 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin cancer using 14765-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 14765-1-AP (MRPL27 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A431 using 14765-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed A431 cells using 14765-1-AP (MRPL27 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, BxPC-3 cells
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:300-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14765-1-AP targets MRPL27 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MRPL27 fusion protein Ag6527
• Full Name: mitochondrial ribosomal protein L27
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 10 kDa
• GenBank Accession Number: BC001066
• Gene Symbol: MRPL27
• Gene ID (NCBI): 51264
• RRID: AB_2145890
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.