MRPL3 Polyclonal antibody

MRPL3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 16584-1-AP

Synonyms

L3mt, MRL3, MRP L3, MRPL3, RPML3

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Validation Data Gallery

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  WB analysis using 16584-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16584-1-AP (MRPL3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 16584-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 16584-1-AP (MRPL3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human cervical cancer using 16584-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 16584-1-AP (MRPL3 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver cancer using 16584-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 16584-1-AP (MRPL3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 16584-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 16584-1-AP (MRPL3 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, HepG2 cells
• Positive IHC detected in: human cervical cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below

Product Information

16584-1-AP targets MRPL3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MRPL3 fusion protein Ag9970
• Full Name: mitochondrial ribosomal protein L3
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC003375
• Gene Symbol: MRPL3
• Gene ID (NCBI): 11222
• RRID: AB_10639509
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MRPL3, also named as 39S ribosomal protein L3, mitochondrial or RPML3, is a 348 amino acid protein, which belongs to the ribosomal protein L3P family. MRPL3 localizes in the Mitochondrion and has an association with combined oxidative phosphorylation deficiency 9. MRPL3 plays role in the mitochondrial proteins biosynthesis and ribosomal structure and biogenesis.

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.; Authors - Elena Lavdovskaia
human	WB	Elife; Defining the interactome of the human mitochondrial ribosome identifies SMIM4 and TMEM223 as respiratory chain assembly factors.; Authors - Sven Dennerlein
human	WB	Hum Mutat; Exome sequencing identifies MRPL3 mutation in mitochondrial cardiomyopathy.; Authors - Galmiche Louise L
human	WB	Biochim Biophys Acta; Mutations in mitochondrial ribosomal protein MRPL12 leads to growth retardation, neurological deterioration and mitochondrial translation deficiency.; Authors - Serre Valérie V
human	WB	STAR Protoc; Protocol to study human mitochondrial ribosome using quantitative density gradient analysis by mass spectrometry and complexome profiling analysis; Authors - Petra Páleníková