MRPL3 Recombinant antibody

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  WB analysis using 85327-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85327-1-RR (MRPL3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85327-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85327-1-RR against Human MRPL3 were performed. The affinity constant is 31.7 nM.

MRPL3 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85327-1-RR

Clone No.242401G9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

MRPL 3, MRP L3, MRL3, L3mt, COXPD9

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Tested Applications

• Positive WB detected in: HepG2 cells, A431 cells, HeLa cells, HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85327-1-RR targets MRPL3 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MRPL3 fusion protein Ag9970
• Full Name: mitochondrial ribosomal protein L3
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 35-39 kDa
• GenBank Accession Number: BC003375
• Gene Symbol: MRPL3
• Gene ID (NCBI): 11222
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P09001
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MRPL3, also named as 39S ribosomal protein L3, mitochondrial or RPML3, is a 348 amino acid protein, which belongs to the ribosomal protein L3P family. MRPL3 localizes in the Mitochondrion and has an association with combined oxidative phosphorylation deficiency 9. MRPL3 plays role in the mitochondrial proteins biosynthesis and ribosomal structure and biogenesis.