MRPL39 Polyclonal antibody

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  WB analysis using 28165-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28165-1-AP (MRPL39 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 28165-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28165-1-AP (MRPL39 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 28165-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28165-1-AP (MRPL39 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 28165-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MRPL39 antibody (28165-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

MRPL39 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 28165-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

MRP L39, Large ribosomal subunit protein mL39, L5mt, L39mt, COXPD59

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse heart tissue, mouse testis tissue, rat testis tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

28165-1-AP targets MRPL39 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag27154

  Product name: Recombinant human MRPL39 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-338 aa of BC107719

  Sequence: MEALAMGSRALRLWLVAPGGGIKWRFIATSPASQLSPTELTEMRNDLFNKEKARQLSLTPRTEKIEVKHVGKTDPGTVFVMNKNISTPYSCAMHLSEWYCRKSILALVDGQPWDMYKPLTKSCEIKFLTFKDCDPGEVNKAYWRSCAMMMGCVIERAFKDEYMVNLVRAPEVPVISGAFCYDVVLDSKLDEWMPTKENLRSFTKDAHALIYKDLPFETLEVEAKVALEIFQHSKYKVDFIEEKASQNPERIVKLHRIGDFIDVSEGPLIPRTSICFQYEVSAVHNLQPTQPSLIRRFQGVSLPVHLRAHFTIWDKLLERSRKMVTEDQSKATEECTST

• Full Name: mitochondrial ribosomal protein L39
• Calculated Molecular Weight: 338 aa, 39 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC107719
• Gene Symbol: MRPL39
• Gene ID (NCBI): 54148
• RRID: AB_2881080
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NYK5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Hum Genet; Deficiency of the mitochondrial ribosomal subunit, MRPL50, causes autosomal recessive syndromic premature ovarian insufficiency; Authors - Shabnam Bakhshalizadeh
human	WB	Signal Transduct Target Ther; The existence of a nonclassical TCA cycle in the nucleus that wires the metabolic-epigenetic circuitry.; Authors - Xujun Liu
human	WB	Hum Mol Genet; Multi-omics identifies large mitoribosomal subunit instability caused by pathogenic MRPL39 variants as a cause of pediatric onset mitochondrial disease; Authors - Sumudu S C Amarasekera