MRPL40 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 83515-4-RR (same clone as 83515-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83515-4-RR (MRPL40 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83515-4-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 83515-4-RR (same clone as 83515-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83515-4-RR (MRPL40 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83515-4-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83515-4-RR (same clone as 83515-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MRPL40 antibody (83515-4-RR, Clone: 240506A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83515-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83515-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83515-4-RR against Human MRPL40 were performed. The affinity constant is 1.56 nM.

MRPL40 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 83515-4-PBS

Clone No.240506A9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

MRP-L40, MRP L40, MRP L22, Large ribosomal subunit protein mL40, L40mt

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Product Information

83515-4-PBS targets MRPL40 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag10341

  Product name: Recombinant human MRPL40 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-206 aa of BC009707

  Sequence: MTASVLRSISLALRPTSGLLGTWQTQLRETHQRASLLSFWELIPMRSEPLRKKKKVDPKKDQEAKERLKRKIRKLEKATQELIPIEDFITPLKFLDKARERPQVELTFEETERRALLLKKWSLYKQQERKMERDTIRAMLEAQQEALEELQLESPKLHAEAIKRDPNLFPFEKEGPHYTPPIPNYQPPEGRYNDITKVYTQVEFKR

• Full Name: mitochondrial ribosomal protein L40
• Calculated Molecular Weight: 206 aa, 24 kDa
• Observed Molecular Weight: 18-24 kDa
• GenBank Accession Number: BC009707
• Gene Symbol: MRPL40
• Gene ID (NCBI): 64976
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9NQ50
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MRPL40, also known as NLVCF and URIM, belongs to the mitochondrion-specific ribosomal protein mL40 family. MRPL40 encodes the mitochondrial ribosomal protein L40 for the larger 39S subunit of mitochondrial ribosomes. MRPL40 is widely expressed in human organs and tissues, including the heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, ovary, small intestine, colon, peripheral blood lymphocyte (PBL), and testis. MRPL40 is also expressed in human fetuses and mouse embryos, suggesting its important role in fetal development (PMID: 36891938).