MRPL48 Polyclonal antibody

MRPL48 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 14677-1-AP

Synonyms

Large ribosomal subunit protein mL48, L48mt, HSPC290, CGI-118, CGI118

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Validation Data Gallery

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  WB analysis of Jurkat using 14677-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14677-1-AP (MRPL48 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14677-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14677-1-AP (MRPL48 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14677-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14677-1-AP (MRPL48 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 14677-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14677-1-AP (MRPL48 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 14677-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14677-1-AP (MRPL48 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 14677-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 14677-1-AP (MRPL48 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IF: See 1 publications below

Product Information

14677-1-AP targets MRPL48 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MRPL48 fusion protein Ag6387
• Full Name: mitochondrial ribosomal protein L48
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 20-21 kDa
• GenBank Accession Number: BC009764
• Gene Symbol: MRPL48
• Gene ID (NCBI): 51642
• RRID: AB_2282151
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MRPL48, also named as CGI-118, or HSPC290 is a 212 amino acid protein, which localizes in the Mitochondrion. MRPL48 is a component of the large subunit of the mitochondrial ribosome, including two RNA molecules and, at least, 70 proteins among which 47 proteins for the large subunit. mitochondrial ribosomal protein helps in protein synthesis within the mitochondrion.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Intellectual disability-associated factor Zbtb11 cooperates with NRF-2/GABP to control mitochondrial function.; Authors - Brooke C Wilson
human	WB	Nucleic Acids Res; Transcription-independent role for human mitochondrial RNA polymerase in mitochondrial ribosome biogenesis.; Authors - Surovtseva Yulia V YV
human	WB	Nucleic Acids Res; MPV17L2 is required for ribosome assembly in mitochondria.; Authors - Ilaria Dalla Rosa
bovine	IF	Int J Mol Sci; Low Expression of Mitofusin 1 Gene Leads to Mitochondrial Dysfunction and Embryonic Genome Activation Failure in Ovine-Bovine Inter-Species Cloned Embryos; Authors - Shanshan Wu
mouse	WB	Cell Rep; MitoRibo-Tag Mice Provide a Tool for In Vivo Studies of Mitoribosome Composition.; Authors - Jakob D Busch
human	WB	Biomedicines; Mitochondrial Ribosome Dysfunction in Human Alveolar Type II Cells in Emphysema; Authors - Loukmane Karim