MRPL52 Polyclonal antibody

MRPL52 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 16800-1-AP

Synonyms

L52mt, MRP L52, MRPL52

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Validation Data Gallery

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  WB analysis of HeLa using 16800-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16800-1-AP (MRPL52 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 16800-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16800-1-AP (MRPL52 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 16800-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16800-1-AP (MRPL52 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 16800-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16800-1-AP (MRPL52 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 16800-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16800-1-AP (MRPL52 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 16800-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16800-1-AP (MRPL52 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 16800-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16800-1-AP (MRPL52 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 16800-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16800-1-AP (MRPL52 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 16800-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16800-1-AP (MRPL52 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human kidney tissue, human spleen tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

16800-1-AP targets MRPL52 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MRPL52 fusion protein Ag10280
• Full Name: mitochondrial ribosomal protein L52
• Calculated Molecular Weight: 123 aa, 14 kDa
• Observed Molecular Weight: 18-21 kDa
• GenBank Accession Number: BC068070
• Gene Symbol: MRPL52
• Gene ID (NCBI): 122704
• RRID: AB_2250853
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB	J Oral Pathol Med; LncRNA LINC00152 promotes oral squamous cell carcinoma growth via enhancing Upstream Transcription Factor 1 mediated Mitochondrial Ribosomal Protein L52 transcription.; Authors - Xiuhe Zou