MRPL54 Polyclonal antibody

MRPL54 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17683-1-AP

Synonyms

MRP-L54, MRP L54, Large ribosomal subunit protein mL54, L54mt

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Validation Data Gallery

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  WB analysis of mouse liver using 17683-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17683-1-AP (MRPL54 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 17683-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17683-1-AP (MRPL54 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin cancer using 17683-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17683-1-AP (MRPL54 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human oesophagus using 17683-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 17683-1-AP (MRPL54 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human oesophagus using 17683-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 17683-1-AP (MRPL54 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 17683-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17683-1-AP (MRPL54 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse liver tissue
• Positive IHC detected in: human skin cancer tissue, human oesophagus tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17683-1-AP targets MRPL54 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MRPL54 fusion protein Ag11782
• Full Name: mitochondrial ribosomal protein L54
• Calculated Molecular Weight: 138 aa, 16 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC065273
• Gene Symbol: MRPL54
• Gene ID (NCBI): 116541
• RRID: AB_2878429
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.