MRPL54 Polyclonal antibody

MRPL54 Polyclonal Antibody for WB, ELISA

Cat No. 17683-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

L54mt, Large ribosomal subunit protein mL54, MRP L54, MRP-L54

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWBIHC

WB analysis using 17683-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17683-1-AP (MRPL54 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human oesophagus using 17683-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 17683-1-AP (MRPL54 Antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human oesophagus using 17683-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 17683-1-AP (MRPL54 Antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive WB detected in

HeLa cells, SKOV-3 cells, OVCAR-3 cells

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

17683-1-AP targets MRPL54 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag11782 Product name: Recombinant human MRPL54 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-138 aa of BC065273 Sequence: MATKRLFGATRTWAGWGAWELLNPATSGRLLARDYAKKPVMKGAKSGKGAVTSEALKDPDVCTDPVQLTTYAMGVNIYKEGQDVPLKPDAEYPEWLFEMNLGPPKTLEELDPESREYWRRLRKQNIWRHNRLSKNKRL Predict reactive species
Full Name	mitochondrial ribosomal protein L54
Calculated Molecular Weight	138 aa, 16 kDa
Observed Molecular Weight	16 kDa
GenBank Accession Number	BC065273
Gene Symbol	MRPL54
Gene ID (NCBI)	116541
RRID	AB_2878429
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q6P161
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.