MRPL9 Polyclonal antibody

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  WB analysis using 15342-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15342-1-AP (MRPL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 15342-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15342-1-AP (MRPL9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15342-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15342-1-AP (MRPL9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15342-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MRPL9 antibody (15342-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

MRPL9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15342-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Synonyms

MRP-L9, MRP L9, Large ribosomal subunit protein bL9m, L9mt

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, MCF-7 cells, PC-3 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

15342-1-AP targets MRPL9 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7545

  Product name: Recombinant human MRPL9 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-267 aa of BC004517

  Sequence: MAAPVVTAPGRALLRAGAGRLLRGGVQELLRPRHEGNAPDLACNFSLSQNRGTVIVERWWKVPLAGEGRKPRLHRRHRVYKLVEDTKHRPKENLELILTQSVENVGVRGDLVSVKKSLGRNRLLPQGLAVYASPENKKLFEEEKLLRQEGKLEKIQTKAGEATVKFLKSCRLEVGMKNNVKWELNPEIVARHFFKNLGVVVAPHTLKLPEEPITRWGEYWCEVTVNGLDTVRVPMSVVNFEKPKTKRYKYWLAQQAAKAMAPTSPQI

• Full Name: mitochondrial ribosomal protein L9
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 26-28 kDa
• GenBank Accession Number: BC004517
• Gene Symbol: MRPL9
• Gene ID (NCBI): 65005
• RRID: AB_2146214
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BYD2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Hum Genet; Deficiency of the mitochondrial ribosomal subunit, MRPL50, causes autosomal recessive syndromic premature ovarian insufficiency; Authors - Shabnam Bakhshalizadeh
human	WB	Front Surg; Classification of the mitochondrial ribosomal protein-associated molecular subtypes and identified a serological diagnostic biomarker in hepatocellular carcinoma; Authors - Cong Xie
human	WB,IHC,IF,CoIP	Int J Mol Sci; Interaction of MRPL9 and GGCT Promotes Cell Proliferation and Migration by Activating the MAPK/ERK Pathway in Papillary Thyroid Cancer; Authors - Hui-Min Zhang
human	WB	Hum Mol Genet; Multi-omics identifies large mitoribosomal subunit instability caused by pathogenic MRPL39 variants as a cause of pediatric onset mitochondrial disease; Authors - Sumudu S C Amarasekera
human	WB	Nat Struct Mol Biol; A roadmap for ribosome assembly in human mitochondria; Authors - Elena Lavdovskaia