MRPS25 Polyclonal antibody

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• KD/KO Validated

MRPS25 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 15277-1-AP

Synonyms

Small ribosomal subunit protein mS25, S25mt, RPMS25, MRP-S25, MRP S25

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Validation Data Gallery

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  WB analysis using 15277-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15277-1-AP (MRPS25 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15277-1-AP

  WB result of MRPS25 antibody (15277-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPS25 transfected HepG2 cells.

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  IP experiment of HEK-293 using 15277-1-AP

  IP result of anti-MRPS25 (IP:15277-1-AP, 4ug; Detection:15277-1-AP 1:500) with HEK-293 cells lysate 1600ug.

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  IHC staining of human intrahepatic cholangiocarcinoma using 15277-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 15277-1-AP (MRPS25 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 15277-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 15277-1-AP (MRPS25 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue, human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IP: See 1 publications below

Product Information

15277-1-AP targets MRPS25 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MRPS25 fusion protein Ag7395
• Full Name: mitochondrial ribosomal protein S25
• Calculated Molecular Weight: 20 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC003590
• Gene Symbol: MRPS25
• Gene ID (NCBI): 64432
• RRID: AB_2180358
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mitochondrial ribosomal protein S25 (MRPS25) belongs to the ribosomal protein S25/L51 family. It is a component of the mitochondrial ribosome small subunit (28S). The variant segregated with the disease and substitutes a highly conserved proline residue with leucine (p.P72L) that, based on the high-resolution structure of the 28S ribosome, is predicted to compromise inter-protein contacts and destabilize the small subunit.

Publications

Species	Application	Title
mouse	WB	PLoS Genet; Mutation in MRPS34 Compromises Protein Synthesis and Causes Mitochondrial Dysfunction.; Authors - Tara R Richman
human	WB	iScience; Supernumerary proteins of the human mitochondrial ribosomal small subunit are integral for assembly and translation; Authors - Taru Hilander
human	WB	Biochem J; Human ERAL1 is a mitochondrial RNA chaperone involved in the assembly of the 28S small mitochondrial ribosomal subunit.; Authors - Dennerlein Sven S
human	WB	Biochim Biophys Acta; Mutations in mitochondrial ribosomal protein MRPL12 leads to growth retardation, neurological deterioration and mitochondrial translation deficiency.; Authors - Serre Valérie V
mouse	WB	Methods Mol Biol; Linear Density Sucrose Gradients to Study Mitoribosomal Biogenesis in Tissue-Specific Knockout Mice.; Authors - Benedetta Ruzzenente
human	WB,IP	Nucleic Acids Res; BOLA3 and NFU1 link mitoribosome iron-sulfur cluster assembly to multiple mitochondrial dysfunctions syndrome; Authors - Hui Zhong