MRPS26 Polyclonal antibody

MRPS26 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 15989-1-AP

Synonyms

C20orf193, GI008, MRP S13, MRP S26, MRPS13, MRPS26, NY BR 87, RPMS13, S13mt, S26mt

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Validation Data Gallery

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  WB analysis of HepG2 using 15989-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15989-1-AP (MRPS26 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15989-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15989-1-AP (MRPS26 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15989-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15989-1-AP (MRPS26 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 15989-1-AP

  IP result of anti-MRPS26 (IP:15989-1-AP, 4ug; Detection:15989-1-AP 1:1000) with HepG2 cells lysate 1085 ug.

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  IHC staining of human liver cancer using 15989-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15989-1-AP (MRPS26 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin cancer using 15989-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15989-1-AP (MRPS26 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 15989-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15989-1-AP (MRPS26 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin cancer using 15989-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15989-1-AP (MRPS26 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, mouse brain tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human skin cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

15989-1-AP targets MRPS26 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MRPS26 fusion protein Ag8797
• Full Name: mitochondrial ribosomal protein S26
• Calculated Molecular Weight: 205 aa, 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC013018
• Gene Symbol: MRPS26
• Gene ID (NCBI): 64949
• RRID: AB_10858479
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	iScience; Supernumerary proteins of the human mitochondrial ribosomal small subunit are integral for assembly and translation; Authors - Taru Hilander
mouse	WB	Life Sci Alliance; Mitochondrial stress response triggered by defects in protein synthesis quality control.; Authors - Uwe Richter
human	WB	Clin Exp Immunol; Cultured lymphocytes' mitochondrial genome integrity is not altered by cladribine; Authors - Elina Järvinen