Validation Data Gallery
|Positive WB detected in||A431 cells, human heart tissue, human skeletal muscle tissue|
|Positive IP detected in||A431 cells|
|Positive IHC detected in||human kidney tissue, human lung tissue, human spleen tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
17280-1-AP targets MRPS27 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MRPS27 fusion protein Ag11047|
|Full Name||mitochondrial ribosomal protein S27|
|Calculated molecular weight||168 aa, 19 kDa, 48 kDa|
|Observed molecular weight||48 kDa|
|GenBank accession number||BC030521|
|Gene ID (NCBI)||23107|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Genes encoding PPR (pentatricopeptide repeat)-containing proteins constitute one of the largest gene families in plants. The majority of these proteins are predicted to target organelles and to bind to RNA. Strikingly, there is a dearth of these proteins in mammals, although genomic searches reveal six candidates, all of which are also predicted to target the mitochondrion. One of these proteins, MRPS27is participated in transcription and translation respectively. [PMID:18939947] MRPS27is a component of the small subunit of the mitochondrial ribosome that is encoded by the nuclear genome [PMID: 11279123].
|Product Specific Protocols|
|WB protocol for MRPS27 antibody 17280-1-AP||Download protocol|
|IHC protocol for MRPS27 antibody 17280-1-AP||Download protocol|
|IF protocol for MRPS27 antibody 17280-1-AP||Download protocol|
|IP protocol for MRPS27 antibody 17280-1-AP||Download protocol|
|Click here to view our Standard Protocols|
The mitochondrial RNA-binding protein GRSF1 localizes to RNA granules and is required for posttranscriptional mitochondrial gene expression.
RNA modification landscape of the human mitochondrial tRNALys regulates protein synthesis.
J Clin Invest
AFG3L2 supports mitochondrial protein synthesis and Purkinje cell survival.
Nucleic Acids Res
The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.
J Cell Biol
Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness.
Proc Natl Acad Sci U S A
Mitochondrial ribosomal protein L12 selectively associates with human mitochondrial RNA polymerase to activate transcription.