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MRPS27 Polyclonal antibody

MRPS27 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17280-1-AP

Synonyms

KIAA0264, MRP S27, MRPS27, S27mt



Tested Applications

Positive WB detected inA431 cells, human heart tissue, human skeletal muscle tissue
Positive IP detected inA431 cells
Positive IHC detected inhuman kidney tissue, human lung tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

17280-1-AP targets MRPS27 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRPS27 fusion protein Ag11047
Full Name mitochondrial ribosomal protein S27
Calculated molecular weight 168 aa, 19 kDa, 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC030521
Gene symbol MRPS27
Gene ID (NCBI) 23107
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Genes encoding PPR (pentatricopeptide repeat)-containing proteins constitute one of the largest gene families in plants. The majority of these proteins are predicted to target organelles and to bind to RNA. Strikingly, there is a dearth of these proteins in mammals, although genomic searches reveal six candidates, all of which are also predicted to target the mitochondrion. One of these proteins, MRPS27is participated in transcription and translation respectively. [PMID:18939947] MRPS27is a component of the small subunit of the mitochondrial ribosome that is encoded by the nuclear genome [PMID: 11279123].

Protocols

Product Specific Protocols
WB protocol for MRPS27 antibody 17280-1-APDownload protocol
IHC protocol for MRPS27 antibody 17280-1-APDownload protocol
IF protocol for MRPS27 antibody 17280-1-APDownload protocol
IP protocol for MRPS27 antibody 17280-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Metab

The mitochondrial RNA-binding protein GRSF1 localizes to RNA granules and is required for posttranscriptional mitochondrial gene expression.

Authors - Antonicka Hana H
humanWB

Nat Commun

RNA modification landscape of the human mitochondrial tRNALys regulates protein synthesis.

Authors - Uwe Richter
mouseWB

J Clin Invest

AFG3L2 supports mitochondrial protein synthesis and Purkinje cell survival.

Authors - Almajan Eva R ER
humanWB

Nucleic Acids Res

The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.

Authors - Elena Lavdovskaia
mouseWB

J Cell Biol

Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness.

Authors - Uwe Richter
humanWB

Proc Natl Acad Sci U S A

Mitochondrial ribosomal protein L12 selectively associates with human mitochondrial RNA polymerase to activate transcription.

Authors - Surovtseva Yulia V YV