|Positive WB detected in||A431 cells, human heart tissue, human skeletal muscle tissue|
|Positive IP detected in||A431 cells|
|Positive IHC detected in||human kidney tissue, human lung tissue, human spleen tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
17280-1-AP targets MRPS27 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MRPS27 fusion protein Ag11047|
|Full Name||mitochondrial ribosomal protein S27|
|Calculated molecular weight||168 aa, 19 kDa, 48 kDa|
|Observed molecular weight||48 kDa|
|GenBank accession number||BC030521|
|Gene ID (NCBI)||23107|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Genes encoding PPR (pentatricopeptide repeat)-containing proteins constitute one of the largest gene families in plants. The majority of these proteins are predicted to target organelles and to bind to RNA. Strikingly, there is a dearth of these proteins in mammals, although genomic searches reveal six candidates, all of which are also predicted to target the mitochondrion. One of these proteins, MRPS27is participated in transcription and translation respectively. [PMID:18939947] MRPS27is a component of the small subunit of the mitochondrial ribosome that is encoded by the nuclear genome [PMID: 11279123].
|Product Specific Protocols|
|WB protocol for MRPS27 antibody 17280-1-AP||Download protocol|
|IHC protocol for MRPS27 antibody 17280-1-AP||Download protocol|
|IF protocol for MRPS27 antibody 17280-1-AP||Download protocol|
|IP protocol for MRPS27 antibody 17280-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Life Sci Alliance
Mitochondrial stress response triggered by defects in protein synthesis quality control.
Methods Mol Biol
Sucrose Gradient Sedimentation Analysis of Mitochondrial Ribosomes.
MRPS27 is a pentatricopeptide repeat domain protein required for the translation of mitochondrially encoded proteins.
J Biol Chem
The Pseudouridine Synthase RPUSD4 is an essential component of Mitochondrial RNA Granules.
J Cell Sci
CLUH couples mitochondrial distribution to the energetic and metabolic status.
Hum Mol Genet
A variant in MRPS14 (uS14m) causes perinatal hypertrophic cardiomyopathy with neonatal lactic acidosis, growth retardation, dysmorphic features and neurological involvement.