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MRPS35 Polyclonal antibody, PBS Only (16457-1-PBS)

Filter: AllWBIPIHCIF/ICC

WB analysis using 16457-1-AP (same clone as 16457-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 16457-1-AP (MRPS35 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16457-1-PBS in a different storage buffer formulation.
WB analysis of human heart using 16457-1-AP (same clone as 16457-1-PBS)
human heart tissue were subjected to SDS PAGE followed by western blot with 16457-1-AP (MRPS35 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16457-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 16457-1-AP (same clone as 16457-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 16457-1-AP (MRPS35 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16457-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 16457-1-AP (same clone as 16457-1-PBS)
IP result of anti-MRPS35 (IP:16457-1-AP, 4ug; Detection:16457-1-AP 1:1000) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 16457-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 16457-1-AP (same clone as 16457-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16457-1-AP (MRPS35 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16457-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 16457-1-AP (same clone as 16457-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16457-1-AP (MRPS35 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16457-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 16457-1-AP (same clone as 16457-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MRPS35 antibody (16457-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16457-1-PBS in a different storage buffer formulation.

MRPS35 Polyclonal antibody, PBS Only

Name: MRPS35 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 16457-1-PBS

MRPS35 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 16457-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

16457-1-PBS targets MRPS35 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9430 Product name: Recombinant human MRPS35 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-323 aa of BC015862 Sequence: MAAAALPAWLSLQSRARTLRAFSTAVYSATPVPTPSLPERTPGNERPPRRKALPPRTEKMAVDQDWPSVYPVAAPFKPSAVPLPVRMGYPVKKGVPMAKEGNLELLKIPNFLHLTPVAIKKHCEALKDFCTEWPAALDSDEKCEKHFPIEIDSTDYVSSGPSVRNPRARVVVLRVKLSSLNLDDHAKKKLIKLVGERYCKTTDVLTIKTDRCPLRRQNYDYAVYLLTVLYHESWNTEEWEKSKTEADMEEYIWENSSSERNILETLLQMKAAEKNMEINKEELLGTKEIEEYKKSVVSLKNEEENENSISQYKESVKRLLNVT Predict reactive species
Full Name	mitochondrial ribosomal protein S35
Calculated Molecular Weight	323 aa, 37 kDa
Observed Molecular Weight	37 kDa
GenBank Accession Number	BC015862
Gene Symbol	MRPS35
Gene ID (NCBI)	60488
RRID	AB_2146521
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P82673
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Mammalian mitochondrial ribosomes (mitoribosomes) are responsible for protein synthesis within the mitochondrion. The mitoribosomes are composed of a 4:1 ratio of protein to RNA, with the proteins forming two subunits, the 28S subunit and the 39S subunit. Across species, the proteins that make up the mitoribosome subunits vary greatly in sequence, preventing easy recognition by sequence homology. MRPS35 (mitochondrial ribosomal protein S35), also known as MDS023, MRPS28 or HDCMD11P, is a 323 amino acid protein that localizes to the mitochondrion, where it exists as a component of the 28S ribosomal subunit and works in conjunction with other MRPs to mediate protein synthesis. Existing as two alternatively spliced isoforms, MRPS35 is encoded by a gene located on human chromosome 10, which houses over 1,200 genes and comprises nearly 4.5% of the human genome. This antibody is a rabbit polyclonal antibody raised against the full length MRPS35 protein. It specifically reacts with the 37kd MRPS35protein.