MRPS9 Polyclonal antibody

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  WB analysis using 16533-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16533-1-AP (MRPS9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 16533-1-AP

  WB result of MRPS9 antibody (16533-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPS9 transfected HEK-293 cells.

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  IHC staining of human intrahepatic cholangiocarcinoma using 16533-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 16533-1-AP (MRPS9 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

MRPS9 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16533-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Small ribosomal subunit protein uS9m, S9mt, RPMS9, MRP-S9, MRP S9

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells, K-562 cells, MCF-7 cells
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

16533-1-AP targets MRPS9 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9809

  Product name: Recombinant human MRPS9 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-396 aa of BC057240

  Sequence: MAAPCVSYGGAVSYRLLLWGRGSLARKQGLWKTAAPELQTNVRSQILRLRHTAFVIPKKNVPTSKRETYTEDFIKKQIEEFNIGKRHLANMMGEDPETFTQEDIDRAIAYLFPSGLFEKRARPVMKHPEQIFPRQRAIQWGEDGRPFHYLFYTGKQSYYSLMHDVYGMLLNLEKHQSHLQAKSLLPEKTVTRDVIGSRWLIKEELEEMLVEKLSDLDYMQFIRLLEKLLTSQCGAAEEEFVQRFRRSVTLESKKQLIEPVQYDEQGMAFSKSEGKRKTAKAEAIVYKHGSGRIKVNGIDYQLYFPITQDREQLMFPFHFVDRLGKHDVTCTVSGGGRSAQAGAIRLAMAKALCSFVTEDEVEWMRQAGLLTTDPRVRERKKPGQEGARRKFTWKKR

• Full Name: mitochondrial ribosomal protein S9
• Calculated Molecular Weight: 396 aa, 46 kDa
• Observed Molecular Weight: 38-45 kDa
• GenBank Accession Number: BC057240
• Gene Symbol: MRPS9
• Gene ID (NCBI): 64965
• RRID: AB_2878272
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P82933
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	J Cell Biol; C17orf80 binds the mitochondrial genome to promote its replication; Authors - Hao Wu
human	WB	Nat Struct Mol Biol; A roadmap for ribosome assembly in human mitochondria; Authors - Elena Lavdovskaia