• Featured Product
  • KD/KO Validated

MSH2 Polyclonal antibody

MSH2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG







Cat no : 15520-1-AP


COCA1, FCC1, hMSH2, HNPCC, HNPCC1, LCFS2, MSH2, MutS protein homolog 2

Tested Applications

Positive WB detected inHeLa cells, Caco-2 cells, SW480 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman stomach cancer tissue, human tonsil tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15520-1-AP targets MSH2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MSH2 fusion protein Ag7835
Full Name mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)
Calculated molecular weight 105 kDa
Observed molecular weight 100 kDa-105 kDa
GenBank accession numberBC021566
Gene symbol MSH2
Gene ID (NCBI) 4436
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MSH2, named for its homologous to the E.coli MutS gene, takes part in DNA mismatch repair(MMR) to maintain the stability of DNA. MSH2 contains a helix-turn-helix domain, which responds for binding to DNA. Also, MSH2 has a direct role in mutation avoidance and micro-satellite stability in cell.


Product Specific Protocols
WB protocol for MSH2 antibody 15520-1-APDownload protocol
IHC protocol for MSH2 antibody 15520-1-APDownload protocol
IF protocol for MSH2 antibody 15520-1-APDownload protocol
IP protocol for MSH2 antibody 15520-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Targeting DNA polymerase β elicits synthetic lethality with mismatch repair deficiency in acute lymphoblastic leukemia

Authors - Ji-Yuan Teng
  • KO Validated

J Immunother Cancer

KALRN mutations promote antitumor immunity and immunotherapy response in cancer.

Authors - Mengyuan Li
humanWB, IHC

Hum Mol Genet

Lack of association of somatic CAG repeat expansion with striatal neurodegeneration in HD knock-in animal models.

Authors - Dazhang Bai
  • KD Validated

Cancer Prev Res (Phila)

Estrogen stimulates the expression of mismatch repair gene hMLH1 in colonic epithelial cells.

Authors - Jin Peng P

Front Oncol

Molecular Classification in Patients With Endometrial Cancer After Fertility-Preserving Treatment: Application of ProMisE Classifier and Combination of Prognostic Evidence.

Authors - Xuting Ran

J Biol Chem

Ectopic expression of human MutS homologue 2 on renal carcinoma cells is induced by oxidative stress with interleukin-18 promotion via p38 mitogen-activated protein kinase (MAPK) and c-Jun N-terminal kinase (JNK) signaling pathways.

Authors - Mo Chen C