MSH3 Polyclonal antibody

MSH3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

6

Reviews (1)

Cat no : 22393-1-AP

Synonyms

mutS homolog 3, Mismatch repair protein 1, hMSH3, DUG, DUC1

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Validation Data Gallery

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  WB analysis using 22393-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22393-1-AP (MSH3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 22393-1-AP

  IP result of anti-MSH3 (IP:22393-1-AP, 4ug; Detection:22393-1-AP 1:1000) with HeLa cells lysate 3600ug.

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  IHC staining of human colon cancer using 22393-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22393-1-AP (MSH3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 22393-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22393-1-AP (MSH3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HT-29 using 22393-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using MSH3 antibody (22393-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HT-29 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below

Product Information

22393-1-AP targets MSH3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MSH3 fusion protein Ag18060
• Full Name: mutS homolog 3 (E. coli)
• Calculated Molecular Weight: 1137 aa, 127 kDa
• Observed Molecular Weight: 127 kDa
• GenBank Accession Number: BC130434
• Gene Symbol: MSH3
• Gene ID (NCBI): 4437
• RRID: AB_11232405
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MSH3 is a DNA mismatch repair (MMR) gene that heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. It undergoes frequent somatic mutation in colorectal cancers (CRCs) with MMR deficiency. MSH3 mismatch repair protein regulates sensitivity to cytotoxic drugs and a histone deacetylase inhibitor in human colon carcinoma cells (PMID: 23724141, PMID:32671096).

Publications

Species	Application	Title
human	WB	Mol Cancer; CircLIFR synergizes with MSH2 to attenuate chemoresistance via MutSα/ATM-p73 axis in bladder cancer.; Authors - Hui Zhang
human	WB	Sci Adv; Deficiency in mammalian STN1 promotes colon cancer development via inhibiting DNA repair; Authors - Dinh Duc Nguyen
human	WB	Hum Mol Genet; Lack of association of somatic CAG repeat expansion with striatal neurodegeneration in HD knock-in animal models.; Authors - Dazhang Bai
	WB	bioRxiv; D-type cyclins regulate DNA mismatch repair in the G1 and S phases of the cell cycle, maintaining genome stability; Authors - Gergely Rona
human	WB	Mol Cell; CDK-independent role of D-type cyclins in regulating DNA mismatch repair; Authors - Gergely Rona
mouse	WB	Prog Neurobiol; Loss of TDP-43 promotes somatic CAG repeat expansion in Huntington's disease knock-in mice; Authors - Dazhang Bai ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Daniela (Verified Customer) (03-17-2022)

In western blot we observed multiple bands, but none at the expected molecular weight. In immunofluorescene, staining was citoplasmatic, while MSH3 is expected to be in the nucleus.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000 for WB; 1:100 for IF
• Cell Tissue Type: Gastric mouse organoids and oesophageal cancer cell line

MSH3 Antibody Western Blot,Immunofluorescence validation (1:1000 for WB; 1:100 for IF dilution) in Gastric mouse organoids and oesophageal cancer cell line (Cat no:22393-1-AP)