Mesothelin Polyclonal antibody

Mesothelin Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15600-1-AP

Synonyms

CAK1, CAK1 antigen, mesothelin, MPF, MPF Mesothelin, cleaved form, MSLN, SMR

---

Validation Data Gallery

• 

  IHC staining of human ovary tumor using 15600-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15600-1-AP (mesothelin antibody at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 15600-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15600-1-AP (mesothelin antibody at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 15600-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15600-1-AP (mesothelin antibody at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 15600-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15600-1-AP (mesothelin antibody at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive IHC detected in: human ovary tumor tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15600-1-AP targets Mesothelin in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Mesothelin fusion protein Ag7981
• Full Name: mesothelin
• Calculated Molecular Weight: 69 kDa
• GenBank Accession Number: BC003512
• Gene Symbol: MSLN
• Gene ID (NCBI): 10232
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mesothelin (MSLN) is a 40kDa GPI-anchored surface glycoprotein that is expressed on pleural and peritoneal mesothelial cells, but not in other non-neoplastic tissues. The mesothelin gene encodes a 69-71kDa precursor protein that is processed to a 40kDa membrane-bound protein termed mesothelin and a 31kDa shed fragment called megakaryocyte-potentiating factor (MPF) that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues (e.g. mesothelium), and is expressed at high levels in mesotheliomas, as well as ovarian, pancreatic, and lung cancers. Mesothelin may be a new target for immunotherapy (PMID: 20922056, 18691948, 15217923).