Mesothelin Monoclonal antibody

Mesothelin Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1









Cat no : 66404-1-Ig


CAK1, CAK1 antigen, mesothelin, MPF, MPF Mesothelin, cleaved form, MSLN, SMR

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

66404-1-Ig targets Mesothelin in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Mesothelin fusion protein Ag23606
Full Name mesothelin
Calculated Molecular Weight 69 kDa
Observed Molecular Weight 69 kDa
GenBank Accession NumberBC003512
Gene Symbol MSLN
Gene ID (NCBI) 10232
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mesothelin (MSLN) is a 40kDa GPI-anchored surface glycoprotein that is expressed on pleural and peritoneal mesothelial cells, but not in other non-neoplastic tissues. The mesothelin gene encodes a 69-71kDa precursor protein that is processed to a 40kDa membrane-bound protein termed mesothelin and a 31kDa shed fragment called megakaryocyte-potentiating factor (MPF) that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues (e.g. mesothelium), and is expressed at high levels in mesotheliomas, as well as ovarian, pancreatic, and lung cancers. Mesothelin may be a new target for immunotherapy (PMID: 20922056, 18691948, 15217923).


Product Specific Protocols
WB protocol for Mesothelin antibody 66404-1-IgDownload protocol
IHC protocol for Mesothelin antibody 66404-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Exp Clin Cancer Res

Mesothelin promotes brain metastasis of non-small cell lung cancer by activating MET

Authors - Shengkai Xia


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Boyan (Verified Customer) (10-05-2021)

This antibody recognized a strong band around 65 kd, which is as shown on the website

  • Applications: Western Blot