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  • KD/KO Validated

Moesin Polyclonal antibody

Moesin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16495-1-AP

Synonyms

MSN, Membrane-organizing extension spike protein



Tested Applications

Positive WB detected inBxPC-3 cells, HeLa cells, Jurkat cells, NIH/3T3 cells, Raji cells, SGC-7901 cells, C6 cells
Positive IHC detected inmouse kidney tissue, human placenta tissue, human skin tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16495-1-AP targets Moesin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Moesin fusion protein Ag9623
Full Name moesin
Calculated Molecular Weight 577 aa, 68 kDa
Observed Molecular Weight 68-70 kDa
GenBank Accession NumberBC017293
Gene Symbol Moesin
Gene ID (NCBI) 4478
RRIDAB_2282374
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Moesin belongs to the ezrin-radixin-moesin (ERM) family of proteins which act as cross-linkers between membrane and actin cytoskeleton. ERM proteins provide structural links to strengthen the cell cortex and facilitate several key cellular processes, including membrane dynamics, substrate adhesion, cell survival, cell adhesion, and motility. The function of ERM proteins is highly reliant on phosphorylation induced conformational changes in response to growth factor, chemokine, and antigen stimulation. This antibody may cross-react with ezrin or radixin with molecular weights around 68-70 kDa.

Protocols

Product Specific Protocols
WB protocol for Moesin antibody 16495-1-APDownload protocol
IHC protocol for Moesin antibody 16495-1-APDownload protocol
IF protocol for Moesin antibody 16495-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Acta Neuropathol Commun

The amyloid plaque proteome in early onset Alzheimer's disease and Down syndrome.

Authors - Eleanor Drummond
humanWB

Int J Mol Sci

Bidirectional Tumor-Promoting Activities of Macrophage Ezrin.

Authors - Krishnendu Khan
  • KD Validated
humanWB

Front Cell Dev Biol

Ectopic Endometrial Cell-Derived Exosomal Moesin Induces Eutopic Endometrial Cell Migration, Enhances Angiogenesis and Cytosolic Inflammation in Lesions Contributes to Endometriosis Progression.

Authors - Maidinaimu Abudula
mouseIF

Am J Pathol

Elevated Expression of Moesin in Muscular Dystrophies.

Authors - Mark Pines
humanIF

Cell Rep Med

Multicellular ecotypes shape progression of lung adenocarcinoma from ground-glass opacity toward advanced stages

Authors - Yulan Deng
humanWB,IP

Drug Resist Updat

Intercellular adhesion molecule-1 suppresses TMZ chemosensitivity in acquired TMZ-resistant gliomas by increasing assembly of ABCB1 on the membrane

Authors - Xin Zhang