Validation Data Gallery
|Positive WB detected in
|BxPC-3 cells, NIH/3T3 cells, HeLa cells, Jurkat cells, Raji cells, SGC-7901 cells, C6 cells
|Positive IHC detected in
|mouse kidney tissue, human placenta tissue, human skin tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Positive FC detected in
|Western Blot (WB)
|WB : 1:5000-1:50000
|IHC : 1:150-1:600
|IF : 1:50-1:500
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
16495-1-AP targets Moesin in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|Moesin fusion protein Ag9623
|Calculated molecular weight
|577 aa, 68 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Moesin belongs to the ezrin-radixin-moesin (ERM) family of proteins which act as cross-linkers between membrane and actin cytoskeleton. ERM proteins provide structural links to strengthen the cell cortex and facilitate several key cellular processes, including membrane dynamics, substrate adhesion, cell survival, cell adhesion, and motility. The function of ERM proteins is highly reliant on phosphorylation induced conformational changes in response to growth factor, chemokine, and antigen stimulation. This antibody may cross-react with ezrin or radixin with molecular weights around 68-70 kDa.
|Product Specific Protocols
|WB protocol for Moesin antibody 16495-1-AP
|IHC protocol for Moesin antibody 16495-1-AP
|IF protocol for Moesin antibody 16495-1-AP
|FC protocol for Moesin antibody 16495-1-AP
|Click here to view our Standard Protocols
Acta Neuropathol Commun
The amyloid plaque proteome in early onset Alzheimer's disease and Down syndrome.
Int J Mol Sci
Bidirectional Tumor-Promoting Activities of Macrophage Ezrin.
Front Cell Dev Biol
Ectopic Endometrial Cell-Derived Exosomal Moesin Induces Eutopic Endometrial Cell Migration, Enhances Angiogenesis and Cytosolic Inflammation in Lesions Contributes to Endometriosis Progression.
Am J Pathol
Elevated Expression of Moesin in Muscular Dystrophies.