Moesin Polyclonal antibody

Moesin Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 16495-1-AP

Synonyms

moesin, MSN



Tested Applications

Positive WB detected inSGC-7901 cells, HeLa cells, NIH/3T3 cells
Positive IHC detected inhuman placenta tissue, human ovary tissue, human skin tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

16495-1-AP targets Moesin in WB, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Moesin fusion protein Ag9623
Full Name moesin
Calculated molecular weight 577 aa, 68 kDa
Observed molecular weight 68-70 kDa
GenBank accession numberBC017293
Gene symbol MSN
Gene ID (NCBI) 4478
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Moesin belongs to the ezrin-radixin-moesin (ERM) family of proteins which act as cross-linkers between membrane and actin cytoskeleton. ERM proteins provide structural links to strengthen the cell cortex and facilitate several key cellular processes, including membrane dynamics, substrate adhesion, cell survival, cell adhesion, and motility. The function of ERM proteins is highly reliant on phosphorylation induced conformational changes in response to growth factor, chemokine, and antigen stimulation. This antibody may cross-react with ezrin or radixin with molecular weights around 68-70 kDa.

Protocols

Product Specific Protocols
WB protocol for Moesin antibody 16495-1-APDownload protocol
IHC protocol for Moesin antibody 16495-1-APDownload protocol
IF protocol for Moesin antibody 16495-1-APDownload protocol
FC protocol for Moesin antibody 16495-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Mol Sci

Bidirectional Tumor-Promoting Activities of Macrophage Ezrin.

Authors - Krishnendu Khan
  • KD Validated
mouseIF

Am J Pathol

Elevated Expression of Moesin in Muscular Dystrophies.

Authors - Mark Pines