Tested Applications
| Positive WB detected in | mouse brain tissue, human heart tissue, mouse heart tissue, rat brain tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17629-1-AP targets MSRB2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11881 Product name: Recombinant human MSRB2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-182 aa of BC018030 Sequence: MARLLWLLRGLTLGTAPRRAVRGQAGGGGPGTGPGLGEAGSLATCELPLAKSEWQKKLTPEQLYVTREKGTEPPFSGIYLNNKEAGMYHCVCCDSPLFSSEKKYCSGTGWPSFSEAHGTSGSDESHTGILRRLDTSLGSARTEVVCKQCEAHLGHVFPDGPGPNGQRFCINSVALKFKPRKH Predict reactive species |
| Full Name | methionine sulfoxide reductase B2 |
| Calculated Molecular Weight | 182 aa, 20 kDa |
| Observed Molecular Weight | 19 kDa |
| GenBank Accession Number | BC018030 |
| Gene Symbol | MSRB2 |
| Gene ID (NCBI) | 22921 |
| RRID | AB_2148380 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y3D2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Methionine sulfoxide reductase B2(MSRB2), is a 182-amino acid protein with an N-terminal mitochondrial targeting signal, a catalytic cysteine, and 2 zinc-coordinating CxxC motifs. MSRB can catalyze the stereospecific reduction of methionine-R-sulfoxides to methionines. MSRB2 protects mitochondrial integrity and cell survival by scavenging reactive oxygen species.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MSRB2 antibody 17629-1-AP | Download protocol |
| WB protocol for MSRB2 antibody 17629-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Antibody: Anti-Methionine Sulfoxide Reductase (Msr) | Catalog No.: 17629-1-AP | Total Citations: 8
Journals: Free Radical Biology and Medicine (2), Frontiers in Pharmacology, Journal of the Science of Food and Agriculture, Food Science and Biotechnology, Molecular Omics, Journal of Food Biochemistry, and Journal of Medical Food.
Research fields: oxidative stress, antioxidant defense pathways (Nrf2-ARE), methionine metabolism, vascular endothelial biology, and proteomics. All applications were Western blot in mouse, human, or rat samples.
| Species | Application | Title |
|---|---|---|
Free Radic Biol Med Response to oxidative stress of AML12 hepatocyte cells with knockout of methionine sulfoxide reductases | ||
Free Radic Biol Med Loss of methionine sulfoxide reductases increases resistance to oxidative stress. | ||
Front Pharmacol 3-Mercaptopyruvate Sulfurtransferase, Not Cystathionine β-Synthase Nor Cystathionine γ-Lyase, Mediates Hypoxia-Induced Migration of Vascular Endothelial Cells.
| ||
J Sci Food Agric l-Methionine activates Nrf2-ARE pathway to induce endogenous antioxidant activity for depressing ROS-derived oxidative stress in growing rats. | ||
Food Sci Biotechnol Methionine strengthens anti-inflammation of rice protein via depressing NF-κB activation and stimulating Msr expression in rats fed cholesterol-enriched diets. | ||
Mol Omics Rbm20 ablation is associated with changes in the expression of titin-interacting and metabolic proteins. |
