RON, MST1R Polyclonal antibody

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  WB analysis of mouse lung using 11053-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 11053-1-AP (RON, MST1R antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 11053-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11053-1-AP (RON, MST1R Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11053-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11053-1-AP (RON, MST1R Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RON, MST1R Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11053-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CD136, CDw136, MSP receptor, MST1R, p185 Ron, Protein tyrosine kinase 8, PTK8, RON, RON, MST1R

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Tested Applications

• Positive WB detected in: mouse lung tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

11053-1-AP targets RON, MST1R in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1537

  Product name: Recombinant human RON, MST1R protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1038-1332 aa of BQ936677 NM_002447

  Sequence: ASFSDSEDESCVPLLRKESIQLRDLDSALLAEVKDVLIPHERVVTHSDRVIGKGHFGVVYHGEYIDQAQNRIQCAIKSLSRITEMQQVEAFLREGLLMRGLNHPNVLALIGIMLPPEGLPHVLLPYMCHGDLLQFIRSPQRNPTVKDLISFGLQVARGMEYLAEQKFVHRDLAARNCMLDESFTVKVADFGLARDILDREYYSVQQHRHARLPVKWMALESLQTYRFTTKSDVWSFGVLLWELLTRGAPPYRHIDPFDLTHFLAQGRRLPQPEYCPDSLYQVMQQCWEADPAVRP

• Full Name: macrophage stimulating 1 receptor (c-met-related tyrosine kinase)
• Calculated Molecular Weight: 152 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BQ936677 NM_002447
• Gene Symbol: RON
• Gene ID (NCBI): 4486
• RRID: AB_874180
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q04912
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RON (also referred to as MST1R, or CD136) is a receptor tyrosine kinase that belongs to the MET proto-oncogene family. The precursor RON is produced as a glycosylated, single chain that is cleaved into a 185-kDa heterodimer of α (35 kDa) and β (150 kDa) subunits connected by a disulfide bond (PMID: 8062829). It is activated upon binding to its ligand, macrophage-stimulating protein (MSP, also known as MST1 and HGFL). RON signalling is essential for embryonic development and regulates many physiological processes including cell survival, migration and differentiation.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Mol Ther Oncolytics; RUNX1/EGFR pathway contributes to STAT3 activation and tumor growth caused by hyperactivated mTORC1.; Authors - Wei Lin
human	WB	Theranostics; Development of dual aptamers-functionalized c-MET PROTAC degraders for targeted therapy of osteosarcoma; Authors - Xuekun Fu