GDF8/Myostatin Polyclonal antibody

• Featured Product
• KD/KO Validated

GDF8/Myostatin Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (5)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

36

Cat no : 19142-1-AP

Synonyms

GDF 8, GDF8, GDF-8, GDF8/Myostatin, MSTN, myostatin

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Validation Data Gallery

• 

  WB analysis using 19142-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 19142-1-AP (GDF8/Myostatin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 19142-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 19142-1-AP (GDF8/Myostatin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 19142-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19142-1-AP (GDF8/Myostatin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 19142-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19142-1-AP (GDF8/Myostatin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 19142-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19142-1-AP (GDF8/Myostatin antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 19142-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19142-1-AP (GDF8/Myostatin antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse brain using 19142-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19142-1-AP (GDF8/Myostatin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human placenta tissue, mouse liver tissue, PC-3 cells, mouse heart tissue
• Positive IHC detected in: human heart tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 31 publications below
• IHC: See 7 publications below
• IF: See 3 publications below

Product Information

19142-1-AP targets GDF8/Myostatin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, pig, zebrafish, bovine, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GDF8/Myostatin fusion protein Ag13605
• Full Name: myostatin
• Calculated Molecular Weight: 375 aa, 43 kDa
• Observed Molecular Weight: 50 and 35 kDa
• GenBank Accession Number: BC074757
• Gene Symbol: MSTN
• Gene ID (NCBI): 2660
• RRID: AB_10638615
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Myostatin, also known as GDF-8, is a member of the TGF-beta superfamily. Myostatin is expressed in the myotome and developing skeletal muscles. Myostatin negatively regulates skeletal muscle growth and development through the regulation of anabolic and catabolic pathways in skeletal muscles. Myostatin immunoreactive bands could be identified at ∼50, 32-35, and 16 kDa, which correspond to the precursor, glycosylated dimmer, and monomer, respectively (PMID: 17711997).

Publications

Species	Application	Title
mouse	WB	J Cachexia Sarcopenia Muscle; Potential therapeutic interventions for chronic kidney disease-associated sarcopenia via indoxyl sulfate-induced mitochondrial dysfunction.; Authors - Yuki Enoki
mouse	WB	Br J Pharmacol; Inhibition of heat shock protein (HSP) 90 reverses signal transducer and activator of transcription (STAT) 3-mediated muscle wasting in cancer cachexia mice.; Authors - Mengyuan Niu
human,rat	WB,IHC,IF	Int J Mol Sci; Renal Ischemia/Reperfusion Early Induces Myostatin and PCSK9 Expression in Rat Kidneys and HK-2 Cells.; Authors - Chiara Barisione ; KD Validated
mouse	WB	iScience; Phosphate depletion in insulin-insensitive skeletal muscle drives AMPD activation and sarcopenia in chronic kidney disease; Authors - Ana Andres-Hernando
mouse	WB	Aging (Albany NY); Vitellogenin 2 promotes muscle development and stimulates the browning of white fat; Authors - Yilei Li
mouse	WB	Sci Rep; Indoxyl sulfate potentiates skeletal muscle atrophy by inducing the oxidative stress-mediated expression of myostatin and atrogin-1.; Authors - Yuki Enoki