MT3 Polyclonal antibody

MT3 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 12179-1-AP

Synonyms

GIF, GIFB, GRIF, Growth inhibitory factor, metallothionein 3, Metallothionein III, MT 3, MT III, MT3

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Validation Data Gallery

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  IHC staining of mouse brain using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12179-1-AP (MT3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12179-1-AP (MT3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12179-1-AP (MT3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12179-1-AP (MT3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12179-1-AP (MT3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12179-1-AP (MT3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12179-1-AP (MT3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12179-1-AP (MT3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12179-1-AP (MT3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12179-1-AP (MT3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12179-1-AP (MT3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12179-1-AP (MT3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12179-1-AP (MT3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12179-1-AP (MT3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12179-1-AP (MT3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12179-1-AP (MT3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12179-1-AP (MT3 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12179-1-AP (MT3 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12179-1-AP (MT3 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive IHC detected in: mouse brain tissue, human brain tissue, human testis tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12179-1-AP targets MT3 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MT3 fusion protein Ag2823
• Full Name: metallothionein 3
• Calculated Molecular Weight: 68 aa, 7 kDa
• GenBank Accession Number: BC013081
• Gene Symbol: MT3
• Gene ID (NCBI): 4504
• RRID: AB_2146645
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MT3(Metallothionein-3) is also names as GIF(Growth inhibitory factor),GIFB inhibits N-glycosylation of IgE-binding factors and that unglycosylated IgE-binding factors selectively suppressed IgE synthesis.The major cell source of GIF is antigen-specific suppressor T(Ts)cell(PMID:10967025).The molecular size of GIF from T cells is 15 kDa, while GIF from macrophages consisted of 40-and 15-kDa molecules(PMID:2417235).