MTCO2 Recombinant antibody, PBS Only

MTCO2 Recombinant Antibody for WB, IP, IF, FC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IP, IF, FC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230448C9

Cat no : 83132-7-PBS

Synonyms

COII, COX2, COXII, cytochrome c oxidase II, Cytochrome c oxidase subunit 2, MT CO2, MTCO2

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Validation Data Gallery

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  WB analysis using 83132-7-RR (same clone as 83132-7-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83132-7-RR (MTCO2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83132-7-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 83132-7-RR (same clone as 83132-7-PBS)

  IP result of anti-MTCO2 (IP:83132-7-RR, 4ug; Detection:83132-7-RR 1:3000) with HeLa cells lysate 1750 ug. This data was developed using the same antibody clone with 83132-7-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83132-7-RR (same clone as 83132-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MTCO2 antibody (83132-7-RR, Clone: 230448C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83132-7-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 83132-7-RR (same clone as 83132-7-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Anti-Human MTCO2 (83132-7-RR, Clone:230448C9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83132-7-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83132-7-PBS targets MTCO2 in WB, IP, IF, FC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: cytochrome c oxidase II
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 23-26 kDa
• GenBank Accession Number: YP_003024029
• Gene Symbol: COX2
• Gene ID (NCBI): 4513
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MTCO2, also named as COII, COXII, and COX2, belongs to the cytochrome c oxidase subunit 2 family. It is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Defects in COX2 are a cause of mitochondrial complex IV deficiency (MT-C4D).