MTCO3 Polyclonal antibody

MTCO3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

20

Cat no : 55082-1-AP

Synonyms

COIII, COXIII, cytochrome c oxidase III, Cytochrome c oxidase subunit 3, MT CO3, MTCO3

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Validation Data Gallery

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  WB analysis using 55082-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55082-1-AP (MTCO3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C2C12 using 55082-1-AP

  C2C12 cells were subjected to SDS PAGE followed by western blot with 55082-1-AP (MTCO3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse cerebellum using 55082-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 55082-1-AP (MTCO3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 55082-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 55082-1-AP (MTCO3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 55082-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 55082-1-AP (MTCO3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 55082-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55082-1-AP (MTCO3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 55082-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55082-1-AP (MTCO3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, C2C12 cells, mouse liver tissue, rat kidney tissue, rat liver tissue
• Positive IHC detected in: mouse cerebellum tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 19 publications below
• IF: See 2 publications below

Product Information

55082-1-AP targets MTCO3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: cytochrome c oxidase III
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 32-35 kDa
• GenBank Accession Number: YP_003024032
• Gene Symbol: MTCO3
• Gene ID (NCBI): 4514
• RRID: AB_2881265
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTCO3 (cytochrome c oxidase subunit III) is a mitochondrial DNA encoded subunit of respiratory Complex IV which is located within the mitochondrial inner membrane and is the final enzyme of the electron transport chain of mitochondrial oxidative phosphorylation. The predicted MW of MTCO3 is 30 kDa, while it had been observed as 23 kDa in some papers (PMID: 17557076).

Publications

Species	Application	Title
mouse	WB	Cell Res; Active RNA interference in mitochondria.; Authors - Kuanxing Gao
mouse	WB	Diabetes; Transketolase Deficiency in Adipose Tissues Protects Mice From Diet-Induced Obesity by Promoting Lipolysis.; Authors - Na Tian
mouse	WB	Int J Mol Sci; Mitochondrial rRNA Methylation by Mettl15 Contributes to the Exercise and Learning Capability in Mice.; Authors - Olga A Averina
human	WB	J Med Genet; COA7 (C1orf163/RESA1) mutations associated with mitochondrial leukoencephalopathy and cytochrome c oxidase deficiency.; Authors - Anabel Martinez Lyons
mouse	IF	Sci China Life Sci; Cholesterol homeostasis regulated by ABCA1 is critical for retinal ganglion cell survival.; Authors - Jialiang Yang
human	WB	Life Sci Alliance; Drosophila MIC10b can polymerize into cristae-shaping filaments; Authors - Till Stephan