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AEG-1/MTDH-Specific Polyclonal antibody

AEG-1/MTDH-Specific Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (1)





Cat no : 13860-1-AP


3D3, 3D3/LYRIC, AEG 1, AEG1, AEG-1/MTDH-Specific, LYRIC, LYRIC/3D3, metadherin, Metastasis adhesion protein, MTDH, Protein LYRIC

Tested Applications

Positive WB detected inMCF-7 cells, A375 cells, HeLa cells, DU 145 cells, HL-60 cells, A549 cells, mouse spleen tissue, PC-3 cells, HepG2 cells, RAW 264.7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue, mouse brain tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13860-1-AP targets AEG-1/MTDH-Specific in WB, IP, IF, RIP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AEG-1/MTDH-Specific fusion protein Ag4840
Full Name metadherin
Calculated Molecular Weight 582 aa, 64 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC045642
Gene Symbol MTDH
Gene ID (NCBI) 92140
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTDH, also known as AEG-1, is a single-pass transmembrane protein with its gene located at chromosome 8q22 , the abnormalities of which have been identified in several tumor types. MTDH is overexpressed in a number of malignancies, such as breast cancer, malignant glioma, and neuroblastoma , and its overexpression is associated with poor clinical outcome. MTDH plays an important role in the regulation of carcinogenesis and tumor progression. This antibody got two bands about 70 kDa and 80 kDa in western blotting, possibly due to posttranslational modification. This antibody is specific to AEG-1/MTDH.


Product Specific Protocols
WB protocol for AEG-1/MTDH-Specific antibody 13860-1-APDownload protocol
IHC protocol for AEG-1/MTDH-Specific antibody 13860-1-APDownload protocol
IF protocol for AEG-1/MTDH-Specific antibody 13860-1-APDownload protocol
IP protocol for AEG-1/MTDH-Specific antibody 13860-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols


humanIF, WB, IP, IHC

J Crohns Colitis

MTDH promotes intestinal inflammation by positively regulating TLR signaling.

Authors - Lijuan Wang


MicroRNA-375 targets AEG-1 in hepatocellular carcinoma and suppresses liver cancer cell growth in vitro and in vivo.

Authors - He X-X XX

Mol Ther

Role of HCP5-miR-139-RUNX1 Feedback Loop in Regulating Malignant Behavior of Glioma Cells.

Authors - Hao Teng

Mol Ther Nucleic Acids

PMID: 28624193

Authors - Yin-Ping Fan


MiR-497 suppresses angiogenesis and metastasis of hepatocellular carcinoma by inhibiting VEGFA and AEG-1.

Authors - Jing-Jun Yan

Int J Cancer

Increased expression of metadherin protein predicts worse disease-free and overall survival in laryngeal squamous cell carcinoma.

Authors - Liu Yong Y


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


eman (Verified Customer) (11-26-2020)

this antibody works very well incubating either at room temperature or overnight2 dilutions are tested 1:1000 and 1:2000IF not tested yet

  • Applications: Western Blot, Immunoprecipitation
  • Primary Antibody Dilution: 1:1000 and 1:2000
  • Cell Tissue Type: Hela cells
AEG-1/MTDH-Specific Antibody Western Blot,Immunoprecipitation validation (1:1000 and 1:2000 dilution) in Hela cells (Cat no:13860-1-AP)