MTERF4 Polyclonal antibody

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  WB analysis using 31892-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31892-1-AP (MTERFD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

MTERF4 Polyclonal Antibody for WB, ELISA

Cat No. 31892-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

MTERFD2, Transcription termination factor 4, mitochondrial, mTERF domain-containing protein 2, MTERF Domain Containing 2 , Mitochondrial transcription termination factor 4

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Tested Applications

• Positive WB detected in: HT-1080 cells, HeLa cells, HepG2 cells, LNCaP cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31892-1-AP targets MTERF4 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MTERF4 fusion protein Ag32334
• Full Name: MTERF domain containing 2
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: NM_182501
• Gene Symbol: MTERFD2
• Gene ID (NCBI): 130916
• RRID: AB_3670135
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q7Z6M4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The MTERF protein family plays a key role in controlling mitochondrial DNA gene expression in mammals. Mitochondrial transcription termination factor 4 (MTERF4, also known as MTERFD2) regulates mitochondrial ribosome biogenesis and translation (PMID: 21531335). It can form a stoichiometric complex with the ribosomal RNA methyl-transferase NSUN4 and is necessary for the recruitment of this factor to the large ribosomal subunit. Loss of MTERF4 leads to defective ribosomal assembly and a drastic reduction in translation (PMID: 21531335).